Analysis of a reaeration model of latent TB reactivation

描述（由申请人提供）：拟议博士后研究的目标是利用体外缺氧模型表征结核分枝杆菌从休眠中重新激活的遗传程序。这些基因重新激活的需求将通过诱变和体外和体内表型评估来测试。这项研究将提高我们对结核分枝杆菌潜伏状态和随后再激活的细菌生理学的理解。该项目的具体目标是：

DESCRIPTION (provided by applicant): The goal of the proposed postdoctoral research is characterize the genetic program of reactivation from dormancy in Mycobacterium tuberculosis using an in vitro hypoxic model. The requirement for these genes in reactivation will be tested by mutagenesis and in vitro and in vivo phenotypic assessment. This investigation will improve our understanding of the bacterial physiology of M. tuberculosis in the latent state and subsequent reactivation. The specific aims of the project are: 1. To use an in vitro model to identify reactivation-specific genes using microarray analysis. 2. To identify genes essential for the transition from hypoxic dormancy to reaerated culture using transposon site hybridization (TraSH). 3. To isolate transposon mutants with insertions into predicted reactivation-specific genes and to test the requirement for these genes for reactivation in vitro and in a mouse model.