Analysis of a reaeration model of laten TB reactivation

潜伏性结核病再激活的再活化模型分析

• 批准号: 7080417
• 负责人: TIGE R RUSTAD
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7080417
• 关键词: Mycobacterium tuberculosis; bacteria infection mechanism; bacterial genetics; disease /disorder model; gene expression; genetic markers; hypoxia; laboratory mouse; latent bacterial disease; microarray technology; postdoctoral investigator; site directed mutagenesis; transposon /insertion element; tuberculosis

DESCRIPTION (provided by applicant): The goal of the proposed postdoctoral research is characterize the genetic program of reactivation from dormancy in Mycobacterium tuberculosis using an in vitro hypoxic model. The requirement for these genes in reactivation will be tested by mutagenesis and in vitro and in vivo phenotypic assessment. This investigation will improve our understanding of the bacterial physiology of M. tuberculosis in the latent state and subsequent reactivation. The specific aims of the project are: 1. To use an in vitro model to identify reactivation-specific genes using microarray analysis. 2. To identify genes essential for the transition from hypoxic dormancy to reaerated culture using transposon site hybridization (TraSH). 3. To isolate transposon mutants with insertions into predicted reactivation-specific genes and to test the requirement for these genes for reactivation in vitro and in a mouse model.

描述（由申请人提供）：拟议的博士后研究的目标是使用体外缺氧模型表征结核分枝杆菌从休眠中重新激活的遗传程序。将通过诱变以及体外和体内表型评估来检测这些基因在再活化中的需求。本研究将加深对M.结核病在潜伏状态和随后的再激活。该项目的具体目标是： 1.利用微阵列分析技术，建立体外模型，鉴定再激活特异性基因。 2.利用转座子位点杂交（TraSH）技术鉴定从低氧休眠到复氧培养的关键基因。 3.分离插入到预测的再激活特异性基因中的转座子突变体，并在体外和小鼠模型中测试这些基因的再激活要求。