Juxtacellular characterization of V1 neurons

(修订摘要)描述：大脑皮层对感知和思维至关重要。了解大脑皮层是如何工作的，是尚未解决的重大科学问题之一。我们一直在研究初级视觉皮质V1，以了解它在视觉中的作用，也因为V1是大脑皮层中最被理解的区域之一。一方面，我们经常对V1中神经元对一系列视觉刺激的放电有详细的了解，这些知识来自细胞外记录。另一方面，通过解剖学研究，我们了解了复杂的神经元结构和V1内以及V1与纹外皮质之间的突触连接模式。为了理解这样一个复杂的神经元网络，建模皮层神经元如何在网络中相互作用是至关重要的。目前，有一个环节缺失，如果存在的话，将使大脑皮层的建模更加逼真：结构和功能之间的联系。在本申请中，我们建议进行实验，使用“松散”贴片方法(JC-LP，细胞旁-松散贴片)扩展细胞旁记录。这项技术的发展将使我们能够在体内对视觉皮质中的神经元进行表征和染色，从而提供功能和结构之间的联系。

(Revised Abstract) Description: The cerebral cortex is vitally important for perception and thought. Understanding how the cortex works is one of the great unsolved scientific problems. We have been studying the primary visual cortex, V1, in order to understand its role in vision and also because the V1 is one of the best-understood regions of the cerebral cortex. On the one hand we often have detailed knowledge of neuronal firing in V1 to an array of visual stimuli, obtained from extracellular recording. On the other hand, from anatomical studies, we know about the intricate neuronal architecture and the patterns of synaptic connections both within V1 and between V1 and extra-striate cortex. In order to understand such a complicated neuronal network, modeling how cortical neurons interact within the network is crucial. At present, there is one link that is missing that if present would enable much more realistic modeling of the cortex: the link between structure and function. In this application we are proposing experiments to pilot an extension of juxtacellular recording using a "loose" patch method (JC-LP, juxtacellular-loose patch). Development of this technique would allow us to characterize and stain neurons in visual cortex in vivo and would thereby provide the link between function and structure.