Antigen Identification in Occult Chorioretinopathies

描述（由申请人提供）：急性带状隐匿性外层视网膜病变、多发性消失白点综合征、急性黄斑神经视网膜病变、急性特发性盲点扩大综合征、多灶性脉络膜炎、点状内层脉络膜病变和弥漫性视网膜下纤维化综合征是一组病因不明的脉络膜视网膜炎症性疾病。这些疾病具有共同的临床特征，受影响的个体可能在其临床过程中从一种病症演变为另一种病症。视力丧失可能从轻微到严重不等。然而，迄今为止，还没有定义疾病亚型的绝对标准，没有诊断疾病或评估预后的可用标志物，也没有有效的治疗方法。 因此，关于这些疾病是否代表炎症性脉络膜视网膜病的相关谱系仍存在争议。 病理标本揭示了 B 细胞为主的炎症浸润和抗体沉积，为我们的假设提供了理论依据，即抗体在这些疾病的发病机制中发挥作用，并且针对触发或传播疾病的视网膜或脉络膜抗原。 为了鉴定隐匿性脉络膜视网膜病 (OC) 特异的抗原，我们将： (1) 鉴定人葡萄膜 cDNA 表达文库和随机肽文库中的克隆，其产物与 OC 患者的血清发生反应； (2) 通过筛选 QC 和对照眼葡萄膜炎患者的血清，确定候选 QC 特异性抗原和肽的疾病特异性； (3) 使用分子生物学技术表征 OC 特异性克隆并研究它们在视网膜和脉络膜中的表达模式。 OC特异性标记物的鉴定将有助于将这些隐匿性炎症性疾病分类为特定的疾病实体，生成生物标记物来诊断临床前和临床疾病，并制定治疗策略以防止受影响个体的视力丧失。

DESCRIPTION (provided by applicant): Acute zonal occult outer retinopathy, multiple evanescent white dot syndrome, acute macular neuroretinopathy, acute idiopathic blind spot enlargement syndrome, multifocal choroiditis, punctuate inner choroidopathy, and diffuse subretinal fibrosis syndrome are a group of chorioretinal inflammatory disorders of unknown etiology. These disorders possess common clinical features, and affected individuals may evolve from one condition to another during their clinical course. Vision loss may vary from mild to severe. To date, however, there are no absolute criteria to define disease subtypes, no available markers to diagnose disease or gauge prognosis, and no effective therapies. As a result, debate remains as to whether these disorders represent a related spectrum of inflammatory chorioretinopathies. Pathologic specimens have revealed a B-cell predominant inflammatory infiltrate with antibody deposition providing a rationale for our hypothesis that antibodies play a role in the pathogenesis of these disorders and are directed against retinal or choroidal antigens that trigger or propagate disease. To identify an antigen specific to occult chorioretinopathies (OC) we will: (1) identify clones in a human uveal cDNA expression Library and a random peptide library whose products react with serum from OC patients; (2) determine the disease-specificity of candidate QC-specific antigens and peptides by screening sera from patients with QC and control ocular uveitides; and (3) characterize, using molecular biologic techniques, OC-specific clones and study their pattern of expression in the retina and choroid. Identification of OC-specific markers will help classify these occult inflammatory disorders as a specific nosologic entity, generate biologic markers to diagnose preclinical and clinical disease, and develop therapeutic strategies to prevent loss of vision in affected individuals.