Novel Locus Required For Photoreceptor Survival

光感受器存活所需的新位点

• 批准号: 6830125
• 负责人: Nansi J. Colley
• 金额: $ 14.55万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6830125
• 关键词: Drosophilidae; calcium channel; disease /disorder etiology; electroretinography; gene expression; genetic disorder; genetic transcription; immunocytochemistry; linkage mapping; macular degeneration; membrane transport proteins; microarray technology; mutant; oxidative stress; quantitative trait loci; retina disorder; retinitis pigmentosa; rhodopsin; sodium channel; visual photoreceptor

DESCRIPTION (provided by applicant): Genetic defects that lead to photoreceptor cell death in age-related macular degeneration (AMD) and retinitis pigmentosa (RP) are highly heterogeneous and now include a list of more than 132 genes. Using Drosophila as a molecular genetic model for unraveling genetic loci in retinal degeneration disorders, such as AMD and RP, we have recently identified an entirely new class of molecules that are critical to photoreceptor viability and cell function. This is a class of sodium dependent symporters or co-transporters that move small molecules across membranes in exchange for sodium. Photoreceptors in Drosophila and humans alike possess several transport systems such as the sodium/calcium (-potassium) exchangers (NCKX) and the ABC transporters. Based on these and other similarities between Drosophila and human visual systems, we propose that the sodium dependent transporters may be critical loci in certain genetically inherited forms of human retinal degeneration. Mutations in the sodium dependent co-transporter lead to photoreceptor pathology and age-related retinal degeneration in Drosophila. In addition, these mutations also cause defects in the molecular weight of rhodopsin. We propose a link between the sodium dependent co-transporter and rhodopsin. In many cases, sodium dependent co-transporters are critical in protecting cells against oxidative damage. Oxidative damage is thought to contribute to AMD and RP as well as diabetic retinopathy. Therefore, sodium dependent transporters may be important loci in some forms of retinal degeneration. Unmasking novel classes of molecules opens up whole new areas of research as well as the development of new therapeutic strategies. Our data continue to support the concept that studies in Drosophila are highly valuable for uncovering the basis of retinal degeneration in humans. Drosophila offers quick insight into new classes of proteins that are strong candidates for photoreceptor function and retinal disease. The high impact and innovation in this application is to utilize the power of Drosophila molecular genetics to provide needed insights into novel loci and mechanisms in retinal degeneration disease. This study provides a rapid translation between Drosophila and humans through collaboration between Drosophila and human genetics laboratories.

描述（由申请人提供）：在年龄相关性黄斑变性（AMD）和视网膜色素变性（RP）中导致光感受器细胞死亡的遗传缺陷是高度异质性的，现在包括超过132个基因的列表。利用果蝇作为揭示视网膜变性疾病（如AMD和RP）遗传位点的分子遗传模型，我们最近发现了一类对光感受器活力和细胞功能至关重要的全新分子。这是一类依赖钠的同向转运体或共转运体，它们通过膜移动小分子以换取钠。果蝇和人类的光感受器都具有多种转运系统，如钠/钙（-钾）交换体（NCKX）和ABC转运体。基于果蝇和人类视觉系统之间的这些和其他相似性，我们提出钠依赖性转运蛋白可能是某些遗传遗传形式的人类视网膜变性的关键位点。钠依赖性共转运体的突变导致果蝇的光感受器病理和与年龄相关的视网膜变性。此外，这些突变还会引起视紫红质分子量的缺陷。我们提出了钠依赖性共转运体和视紫红质之间的联系。在许多情况下，钠依赖性共转运蛋白在保护细胞免受氧化损伤方面起着至关重要的作用。氧化损伤被认为有助于AMD和RP以及糖尿病视网膜病变。因此，钠依赖性转运蛋白可能是某些形式视网膜变性的重要位点。揭示新的分子类别开辟了全新的研究领域以及新的治疗策略的发展。我们的数据继续支持这样一个概念，即果蝇的研究对于揭示人类视网膜变性的基础非常有价值。果蝇提供了快速洞察新类别的蛋白质，这些蛋白质是光感受器功能和视网膜疾病的强有力候选者。该应用的高影响力和创新在于利用果蝇分子遗传学的力量，为视网膜变性疾病的新位点和机制提供所需的见解。本研究通过果蝇和人类遗传学实验室之间的合作，提供了果蝇和人类之间的快速翻译。