A New Method for Retinal Imaging

视网膜成像的新方法

• 批准号: 6936507
• 负责人: ALAN D MARMORSTEIN
• 金额: $ 15.05万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-07 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6936507
• 关键词: aging; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; eye; eye disorder diagnosis; eye fundus photography; fluorescence; human tissue; macular degeneration; patient oriented research; postmortem; retinal pigment epithelium; retinography

DESCRIPTION (provided by applicant): Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in the western world affecting nearly 30% of those over the age of 75. The only accepted risk factors for AMD are age, race, and smoking. AMD alters the quality of life of those affected by causing a debilitating loss of central vision. Currently diagnosis of AMD at its early stages can be difficult. This has led to problems in the development of drugs to treat the disease since clinical trials must rely on following the slow progression of the disease in individuals that are already demonstrating some loss of visual acuity. We have recently found that Bruch's membrane and sub-retinal pigment epithelium (RPE) deposits characteristic of AMD exhibit a unique autofluorescence spectrum that can be excited with light between 360 and 490nm. When compared with the intensity of autofluorescence elicited from RPE lipofuscin, these emissions were significantly elevated in eyes from donors with AMD versus healthy donor eyes. The change in the ratios of these emissions (Bruch's membrane and sub-RPE deposits vs. lipofuscin) correlating with disease suggests that the same principles that allow quantitative microscopy using fluorescence ratiometry could be applied to fundus photography. The goal of the work outlined in this proposal is to design, build, and test a device for fundus fluorescence ratiometry, and to determine the efficacy of this device in the early diagnosis of retinal lesions that are risk factors for AMD. To accomplish this we will perform a series of 3 specific aims. In the first aim we will modify a standard clinical fundus camera for fundus fluorescence ratiometry. In specific aim 2 we will determine ideal excitation and emission wavelengths for fundus fluorescence ratiometry. This will be accomplished by testing a variety of excitation and emission wavelengths using the modified camera on postmortem human donor eyes. In specific aim 3 we will determine whether fundus fluorescence ratiometry shows promise for the diagnosis of lesions associated with AMD. This will be accomplished using eyes from donors with AMD. Specifically we will test the efficacy of this device in identifying basal deposits. Basal deposits are specific retinal lesions that are not visible during a clinical fundus exam. One form of basal deposit, basal linear deposits, are more common in AMD eyes than age-matched controls. A positive outcome to this study would warrant future clinical trials.

描述（由申请人提供）：视网膜相关性黄斑变性（AMD）是西方世界不可逆失明的主要原因，影响近30%的75岁以上人群。 唯一公认的AMD风险因素是年龄，种族和吸烟。 AMD通过引起中心视力的衰弱性丧失而改变受影响者的生活质量。 目前在早期阶段诊断AMD可能很困难。 这导致了治疗该疾病的药物开发中的问题，因为临床试验必须依赖于已经表现出一些视力丧失的个体中疾病的缓慢进展。 我们最近发现，AMD的特征性布鲁赫膜和视网膜下色素上皮（RPE）沉积物表现出独特的自体荧光光谱，其可以用360和490 nm之间的光激发。 当与RPE脂褐素引起的自体荧光强度相比时，这些发射在来自AMD供体的眼睛中与健康供体眼睛相比显著升高。 与疾病相关的这些发射（布鲁赫膜和亚RPE沉积物与脂褐质）的比率的变化表明，允许使用荧光比率测定法的定量显微术的相同原理可以应用于眼底摄影。 本提案中概述的工作目标是设计、构建和测试眼底荧光比率测量装置，并确定该装置在早期诊断AMD风险因素视网膜病变中的疗效。为了实现这一目标，我们将执行一系列的3个具体目标。 在第一个目标中，我们将修改一个标准的临床眼底照相机眼底荧光比率。 在具体目标2中，我们将确定理想的激发和发射波长的眼底荧光比率。 这将通过在死后人类供体眼睛上使用改进的相机测试各种激发和发射波长来实现。 在具体目标3中，我们将确定眼底荧光比率测定法是否有希望用于诊断与AMD相关的病变。 这将使用来自患有AMD的供体的眼睛来完成。 具体而言，我们将测试该设备在识别基底沉积物方面的功效。 基底沉积物是临床眼底检查中不可见的特定视网膜病变。基底存款的一种形式，基底线性沉积物，在AMD眼中比年龄匹配的对照组更常见。 这项研究的积极结果将保证未来的临床试验。