Establishing a synthetic biology toolbox for biosensor development in important probiotic host organisms.
建立用于重要益生菌宿主生物体生物传感器开发的合成生物学工具箱。
基本信息
- 批准号:2602359
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:英国
- 项目类别:Studentship
- 财政年份:2021
- 资助国家:英国
- 起止时间:2021 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
The fundamental impact of microbiome-based bacteria upon human health is increasingly understood. Dysbiosis is an imbalance of microbiome bacterial constituents that is strongly linked to the pathology of a wide range of conditions. This project will develop new approaches to rebalance the microbiome to treat and prevent such diseases. This project will engineer probiotic organisms to detect metabolic changes that are characteristic of pathologic states and trigger the deployment of appropriate treatments at the site of interest. This will require the development of engineering tools for probiotic lactic acid bacteria, as well as bioinformatics approaches to identify new sensing components required to create the required biosensors.We currently lack the tools necessary to detect and selectively remove bacterial constituents of the microbiome that cause dysbiosis. This limits therapeutic intervention. Novel, next-generation therapeutic bacteria (NTB) capable of sensing and precisely resolving dysbiosis represent a new frontier in the treatment of microbiome related disorders. This project will develop key dysbiosis-sensing components to create new bacterial therapies, by creating a biosensing toolbox enabling genetic engineering of lactic acid bacteria that can sense and react to metabolic changes characteristic of microbiome dysbiosis. Ultimately, these bacterial biosensors will control the deployment of specific bactericidal payloads into the microbiome by NTB.A bioinformatics approach will be taken to identify new sensing components that govern transcriptional regulation in response to external signals. These will then be repurposed as biosensors for the detection of the state of the microbiome. To better be able to engineer probiotic bacteria, a synthetic biology toolbox will be created for suitable hosts. For therapeutic use in the human microbiome, lactic acid bacteria such as Lactobacillus spp. are an ideal choice given their Generally Regarded As Safe (GRAS) status. However, few characterised promoters, ribosome binding sites, terminators and vectors are described for Lactobacillus spp., addressing this need will therefore transform our ability to reliably engineer these types of organism.
人们越来越了解以微生物群为基础的细菌对人类健康的根本影响。微生物失调是一种微生物群细菌成分的失衡,与一系列疾病的病理密切相关。该项目将开发新的方法来重新平衡微生物群,以治疗和预防此类疾病。该项目将对益生菌进行改造,以检测具有病理状态特征的代谢变化,并在感兴趣的部位触发适当治疗的部署。这将需要开发益生菌乳酸菌的工程工具,以及识别创建所需生物传感器所需的新传感组件的生物信息学方法。目前,我们缺乏必要的工具来检测和选择性地去除导致微生物组失调的细菌成分。这限制了治疗干预。新型的下一代治疗性细菌(NTB)能够感知和精确地解决生态失调,是治疗微生物组相关疾病的新前沿。该项目将开发关键的微生物失调传感组件,通过创建一个生物传感工具箱,使乳酸菌的基因工程能够感知微生物组失调的代谢变化并做出反应,从而创造新的细菌疗法。最终,这些细菌生物传感器将控制NTB将特定杀菌有效载荷部署到微生物组中。我们将采用生物信息学的方法来识别新的传感组件,这些组件管理对外部信号的转录调控。然后,这些将被重新用作生物传感器,用于检测微生物组的状态。为了更好地设计益生菌,将为合适的宿主创建合成生物学工具箱。乳酸菌等乳酸菌在人体微生物群中的治疗用途。考虑到它们通常被认为是安全的(GRAS)状态,这是一个理想的选择。然而,鲜有乳杆菌特有的启动子、核糖体结合位点、终止子和载体被描述,满足这一需求将改变我们可靠地设计这些类型生物的能力。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
其他文献
吉治仁志 他: "トランスジェニックマウスによるTIMP-1の線維化促進機序"最新医学. 55. 1781-1787 (2000)
Hitoshi Yoshiji 等:“转基因小鼠中 TIMP-1 的促纤维化机制”现代医学 55. 1781-1787 (2000)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
LiDAR Implementations for Autonomous Vehicle Applications
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
吉治仁志 他: "イラスト医学&サイエンスシリーズ血管の分子医学"羊土社(渋谷正史編). 125 (2000)
Hitoshi Yoshiji 等人:“血管医学与科学系列分子医学图解”Yodosha(涉谷正志编辑)125(2000)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Effect of manidipine hydrochloride,a calcium antagonist,on isoproterenol-induced left ventricular hypertrophy: "Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,K.,Teragaki,M.,Iwao,H.and Yoshikawa,J." Jpn Circ J. 62(1). 47-52 (1998)
钙拮抗剂盐酸马尼地平对异丙肾上腺素引起的左心室肥厚的影响:“Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('', 18)}}的其他基金
An implantable biosensor microsystem for real-time measurement of circulating biomarkers
用于实时测量循环生物标志物的植入式生物传感器微系统
- 批准号:
2901954 - 财政年份:2028
- 资助金额:
-- - 项目类别:
Studentship
Exploiting the polysaccharide breakdown capacity of the human gut microbiome to develop environmentally sustainable dishwashing solutions
利用人类肠道微生物群的多糖分解能力来开发环境可持续的洗碗解决方案
- 批准号:
2896097 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
A Robot that Swims Through Granular Materials
可以在颗粒材料中游动的机器人
- 批准号:
2780268 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Likelihood and impact of severe space weather events on the resilience of nuclear power and safeguards monitoring.
严重空间天气事件对核电和保障监督的恢复力的可能性和影响。
- 批准号:
2908918 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Proton, alpha and gamma irradiation assisted stress corrosion cracking: understanding the fuel-stainless steel interface
质子、α 和 γ 辐照辅助应力腐蚀开裂:了解燃料-不锈钢界面
- 批准号:
2908693 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Field Assisted Sintering of Nuclear Fuel Simulants
核燃料模拟物的现场辅助烧结
- 批准号:
2908917 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Assessment of new fatigue capable titanium alloys for aerospace applications
评估用于航空航天应用的新型抗疲劳钛合金
- 批准号:
2879438 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Developing a 3D printed skin model using a Dextran - Collagen hydrogel to analyse the cellular and epigenetic effects of interleukin-17 inhibitors in
使用右旋糖酐-胶原蛋白水凝胶开发 3D 打印皮肤模型,以分析白细胞介素 17 抑制剂的细胞和表观遗传效应
- 批准号:
2890513 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Understanding the interplay between the gut microbiome, behavior and urbanisation in wild birds
了解野生鸟类肠道微生物组、行为和城市化之间的相互作用
- 批准号:
2876993 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
相似国自然基金
近空间飞行器载MIMO SAR高分辨率、宽测绘带遥感成像机理与方法
- 批准号:41101317
- 批准年份:2011
- 资助金额:25.0 万元
- 项目类别:青年科学基金项目
基于大机动运动平台的特定目标多极化成像与匹配技术研究
- 批准号:11176022
- 批准年份:2011
- 资助金额:46.0 万元
- 项目类别:联合基金项目
相似海外基金
Conference: 2024 Mammalian Synthetic Biology Workshop
会议:2024年哺乳动物合成生物学研讨会
- 批准号:
2412586 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Applying synthetic biology to the development of in vivo technologies for the monitoring and control of vector-borne diseases.
应用合成生物学来开发用于监测和控制媒介传播疾病的体内技术。
- 批准号:
BB/Y008340/1 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Research Grant
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Conference: 2024 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference
会议:2024年合成生物学:工程、进化和设计(SEED)会议
- 批准号:
2413726 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
CAREER: Synthetic Biology to Understand and Harness Plant Enzyme Complexes for Natural Product Synthesis in Yeast
职业:合成生物学,了解和利用植物酶复合物在酵母中合成天然产物
- 批准号:
2338009 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Continuing Grant
Transition: Metabolomics-driven understanding of rules that coordinate metabolic responses and adaptive evolution of synthetic biology chassis
转变:代谢组学驱动的对协调代谢反应和合成生物学底盘适应性进化的规则的理解
- 批准号:
2320104 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Standard Grant
Conference: 2023 Mammalian Synthetic Biology Workshop
会议:2023哺乳动物合成生物学研讨会
- 批准号:
2330030 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Standard Grant
A synthetic biology approach to unlocking the role of the ribosome in cell competition
揭示核糖体在细胞竞争中的作用的合成生物学方法
- 批准号:
489621 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Operating Grants
A UK-Japan partnership for synergising synthetic biology with systems biology.
英国-日本合作伙伴关系,旨在协同合成生物学与系统生物学。
- 批准号:
BB/X018318/1 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Research Grant
Synthetic biology approaches to construct metal analogues of vitamin B12 to act as anti-microbial and imaging agents for health applications.
利用合成生物学方法构建维生素 B12 的金属类似物,作为健康应用的抗菌剂和显像剂。
- 批准号:
2881504 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Studentship