Pyk2 Signaling In Intestinal Epithelial Cells

肠上皮细胞中的 Pyk2 信号转导

• 批准号: 6825763
• 负责人: STEVEN S WU
• 金额: $ 12.18万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-15 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6825763
• 关键词: Pyk2; Signaling; Intestinal; Epithelial; Cells

CONTINUATIONPAGE Principal Investigator/PrograrnDirector (Last, first,middle): Wu, Steven Shi-fong 5. Environmental and Institutional Commitmentto the Candidate a. Description of the Institutional Environment In addition to direct mentoring from Dr. Enrique Rozengurt in the fields of signal transduction and growth regulation, Dr. Wu will benefit from the advice and guidance of his ScientificAdvisory Committee, consisting of myself, Dr. Martin Martin, Associate Professor in the Division of Gastroenterology, and Dr. Sherin Devaskar, Professor, Chiefof the Division ofNeonatology, and Vice Chair for Research. We are all very committed to mentoring fellows and young faculty. Dr. Linda McCabe and I have been offering an annual course, How to Succeed in Academics, since 1991,and we have published a book of the sametitle. We also initiated a Leadership Workshop beginning in 1996. We are absolutely committed to mentoring and Dr. Wu's committee exemplifies individuals for whom this is a central value. Since being recruited to the UCLA faculty in 1997, Dr. Rozengurt has been the recipient of three NIDDK R01 grants and a section of an NCI P50 SPORE institutional project grant, and has established an outstanding and productive laboratory group within the Division of Digestive Diseases, Department of Medicine. In addition to Dr. Wu, this laboratory group includes four graduate students of whom three are post-M.D. fellows, eight postdoctoral Ph.D. fellows, three part-time undergraduate students, one research associate, and one technician. The laboratory covers 5,000 square feet on the first floor of Warren Hall, which houses research space for the Department of Nutrition and several divisions of the Department of Medicine. The laboratory is fully equipped for signal transduction research with two cell culture hoods, multiple incubators, refrigerators, and freezers including liquid nitrogen cell storage, as well as equipment for protein and nucleotide electrophoresis and gel transfer. There is a spectrophotometer for measuring intracellular calcium flux, a darkroom with an X-ray film developing machine, and a separate room for radioactive work and storage, and including a liquid scintillation counter. The laboratory shares access within the building to a high resolution videomicroscopy system and elsewhere on the UCLA campus to the core resources of the Molecular Biology Institute, including confocal microscopy equipment, and of the CURE/Digestive Diseases Research Center. As an important part of his career development and training, Dr. Wu has previously attended our two- day workshop on How to Succeed in Academics, and will attend a future one-day workshop on Academic Leadership, in addition to a series of weekly noon conferences developed by Dr. Devaskar. These conferences rotate between the following topics: Didactic training in establishing a successful research career; a work-in-progress seminar with presentations by fellows andjunior faculty; a departmental research seminar presented by senior faculty; and a pediatric researchjournal club. He will also attend a weekly Division of Digestive Diseasesjournal club and a biweekly Signal Transduction Group seminar. He will enroll in the graduate level courses outlined in the Candidate Background section of his proposal, which will enrich his understanding of molecular biology and genetics. He will continue to present his research in lab meetings, departmental meetings, and at national meetings such as those of the American Gastroenterological Association (Digestive Disease Week) and ofthe North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition (NASPGHAN). This combination of resources, from both Dr. Rozengurt's laboratory and the Department of Pediatrics, will provide the intellectual and physical resources for a supportive and stimulatingtraining program for Dr. Wu, as have been provided for many of our previous trainees who are successful in academic medicine. In my experience with previous trainees at UCLA and other institutions,trainees with Dr. Wu's background, skills, and motivation have become truly superb physician-scientists. A KO8 grant will be instrumental in launching his academic career. As Chair of the Department of Pediatrics, I will continue to protect his research time at 80% throughout his five-year tenure on the K08, and beyond, if he elects to remain at UCLA. I am confident that Dr. Wu will be productive and innovative in his approach to research, and will continue to develop into an outstanding independent physician-scientist in the field ofpediatric gastroenterology. PHS 398 (Rev. 4/98) Page 21 Use %-inch MARGINS. Number pages consecutively at the bottomthroughout the application. Domusesuffixes such as 3a, 3b. ========================================Section End===========================================

续页 主要研究者/项目负责人（最后一名、第一名、中间名）： 吴世芳 5.对候选人的环境和制度承诺。机构环境描述 除了在信号转导和生长调节领域得到Enrique Rozengurt博士的直接指导外，Wu博士还将受益于他的科学咨询委员会的建议和指导，该委员会由我本人、胃肠病学系副教授Martin Martin博士和新生儿学系主任兼研究副主席Sherin Devaskar博士组成。 我们都非常致力于指导研究员和年轻的教师。 琳达·麦凯布博士和我从1991年开始每年都开设一门课程，名为《如何在学术上取得成功》，我们还出版了一本同名的书。我们还从1996年开始举办领导力讲习班。 我们绝对致力于指导和吴博士的委员会认证的个人，这是一个核心价值观。 自1997年被招募到加州大学洛杉矶分校的教师，博士Rozengurt一直是三个NIDDK R 01赠款和NCI P50 SPORE机构项目赠款的一部分的收件人，并建立了消化疾病，医学系内的部门一个杰出的和富有成效的实验室组。 除吴博士外，这个实验组还包括四名研究生，其中三名是医学博士。研究员，8名博士后。研究员，三名兼职本科生，一名研究助理和一名技术员。 该实验室占地5,000平方英尺，位于沃伦大厅的一楼，为营养系和医学系的几个部门提供研究空间。 该实验室设备齐全，可用于信号转导研究，包括两个细胞培养罩，多个孵育器，冰箱和冰柜，包括液氮细胞储存，以及蛋白质和核苷酸电泳和凝胶转移设备。 有一个用于测量细胞内钙通量的分光光度计，一个带有X射线胶片显影机的暗室，以及一个用于放射性工作和储存的单独房间，并包括一个液体闪烁计数器。 该实验室在建筑物内共享高分辨率视频显微镜系统，并在加州大学洛杉矶分校校园的其他地方共享分子生物学研究所的核心资源，包括共聚焦显微镜设备和CURE/消化系统疾病研究中心。 作为他职业发展和培训的重要组成部分，吴博士之前参加了我们为期两天的关于如何在学术上取得成功的研讨会，并将参加未来为期一天的关于学术领导力的研讨会，以及由Devaskar博士开发的一系列每周中午会议。 这些会议在以下主题之间轮换：建立成功的研究生涯的教学培训;由研究员和初级教师介绍的正在进行的研讨会;由高级教师介绍的部门研究研讨会;和儿科研究期刊俱乐部。 他还将参加每周一次的消化疾病杂志俱乐部和双周一次的信号转导小组研讨会。 他将报名参加在他的建议，这将丰富他的分子生物学和遗传学的理解候选人背景部分概述的研究生水平的课程。他将继续在实验室会议，部门会议和国家会议上介绍他的研究，如美国胃肠病协会（消化疾病周）和北美儿科胃肠病，肝病和营养学会（NASPGHAN）。 Rozengurt博士的实验室和儿科部门的资源组合将为吴博士的支持和刺激培训计划提供智力和体力资源，正如我们以前为许多在学术医学方面取得成功的学员提供的那样。 根据我以前在加州大学洛杉矶分校和其他机构实习的经验，具有吴博士背景、技能和动力的实习生已经成为真正优秀的医学科学家。 KO 8赠款将有助于启动他的学术生涯。 作为儿科系主任，如果他选择留在加州大学洛杉矶分校，我将继续保护他在K 08的五年任期内80%的研究时间。 我相信吴博士将在他的研究方法上富有成效和创新，并将继续发展成为儿科胃肠病学领域杰出的独立医生科学家。 PHS 398（Rev.4/98） 第21页使用%-英寸边距。在整个应用程序的底部连续编号页面。 Domusesufixes，如3a，3b。==========================================================