Elucidating the role of Fam20C in microglia cells during homeostasis and neurodevelopment

该项目旨在揭示分泌激酶Fam 20 C在小胶质细胞中的功能;以及FAM 20 C的破坏如何导致脑内稳态和神经发育中小胶质细胞功能失调。这个建议是在MRC的职权范围内，进一步了解神经科学的分子和细胞机制。本项目将采用跨学科的方法，通过磷酸化蛋白质组学、生物化学、离体小胶质细胞和小鼠模型的基因组编辑来确定Fam 20 C的分子、细胞和神经小胶质细胞功能。本研究的潜在成果是提高我们对小胶质细胞中磷酸化分泌组如何在神经发育和疾病背景下调节小胶质细胞功能的理解。

This project aims to uncover the function of the secreted kinase, Fam20C, in microglia cells; and how disruption of FAM20C may lead to dysregulation of microglial function in brain homeostasis and neurodevelopment. This proposal is within the MRC's remit of furthering knowledge on molecular and cellular mechanisms in neuroscience. This project will use an interdisciplinary approach by employing phosphoproteomics, biochemistry, genome editing in ex-vivo microglia and a mouse model to determine Fam20C molecular, cellular, and neurological microglial functions.The potential outcome of this study is an improvement on our understanding of how the phosphor-secretome in microglia cells regulate microglial function in the context of neurodevelopment and disease.