Transmission Electron Microscope
透射电子显微镜
基本信息
- 批准号:6813400
- 负责人:
- 金额:$ 200万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-07-15 至 2005-07-14
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): Molecular electron cryo-microscopy (cryoEM) is at the same stage of development today as macromolecular x-ray crystallography was in 1980. We are entering an exponential growth phase of this technology accompanied by significant expansion in the number of practitioners and applications. Publications describing the utilization molecular cryoEM in combination with crystallography have grown dramatically and they have revealed exceptionally interesting features of mechanistic biology. Molecular cryoEM fills a unique niche between high resolution cytological EM and crystallography. Examining specimens not immobilized in a crystal lattice has allowed the characterization of dynamic features of macromolecular assemblies impossible to characterize by crystallography and to determine sub nanometer structures of species too large or too heterogeneous to be crystallized. A remarkably powerful strategy has been to determine high-resolution structures of the components of a macromolecular complex by crystallography or NMR followed by the construction of a pseudo atomic resolution model based on the cryoEM reconstruction of the intact organization. At resolutions currently achievable with high voltage FEG instruments, the precision of this modeling into cryoEM density is exceptionally high. This application requests partial support from NIH for a 300KV electron microscope equipped with a field emission gun, a helium cryo stage and a 4k by 4k slow scan CCD. This instrument will fill a gap that exists on the Torrey Pines Mesa in the atoms to cells continuum of structural studies that is the backbone of the world-class biological science produced by the participating institutions; University of California, San Diego; The Burnham Institute and The Scripps Research Institute. Projects proposed for high resolution work with this instrument include molecular motors, membrane proteins and their interactions, complexes controlling intercellular communication and virus assembly and maturation. In addition, software and hardware developed for high throughput EM data collection will be implemented on the requested microscope as it is perfected. These are all well funded programs that have benefited from moderate resolution studies and will immediately utilize the new technology when it is available. We anticipate that a broad range of projects from the Torrey Pines Mesa will find this technology useful as investigators not currently directly involved with molecular cryoEM are introduced to results emerging from its use.
描述(由申请人提供):如今,分子电子冷冻显微镜 (cryoEM) 与 1980 年的大分子 X 射线晶体学处于同一发展阶段。我们正在进入该技术的指数增长阶段,同时伴随着从业者和应用数量的显着扩展。描述分子冷冻电镜与晶体学结合应用的出版物数量急剧增加,它们揭示了机械生物学异常有趣的特征。分子冷冻电镜填补了高分辨率细胞电镜和晶体学之间的独特空白。通过检查未固定在晶格中的样品,可以表征晶体学无法表征的大分子组装体的动态特征,并确定太大或太异质而无法结晶的物质的亚纳米结构。一种非常强大的策略是通过晶体学或核磁共振确定大分子复合物组分的高分辨率结构,然后基于完整组织的冷冻电镜重建构建伪原子分辨率模型。在目前高压 FEG 仪器可实现的分辨率下,冷冻电镜密度建模的精度非常高。该申请请求 NIH 部分支持配备场发射枪、氦冷冻台和 4k x 4k 慢扫描 CCD 的 300KV 电子显微镜。该仪器将填补多利松台地在原子到细胞结构研究连续体方面存在的空白,这是参与机构产生的世界级生物科学的支柱;加州大学圣地亚哥分校;伯纳姆研究所和斯克里普斯研究所。使用该仪器进行高分辨率工作的拟议项目包括分子马达、膜蛋白及其相互作用、控制细胞间通讯以及病毒组装和成熟的复合物。此外,为高通量 EM 数据收集而开发的软件和硬件将在完善后在所需的显微镜上实施。这些都是资金充足的项目,受益于中等分辨率的研究,并将在新技术可用时立即利用它。我们预计 Torrey Pines Mesa 的广泛项目将发现这项技术很有用,因为目前未直接参与分子冷冻电镜的研究人员将了解其使用所产生的结果。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Enhanced magnetic resonance contrast of Fe₃O₄ nanoparticles trapped in a porous silicon nanoparticle host.
- DOI:10.1002/adma.201101877
- 发表时间:2011-09-22
- 期刊:
- 影响因子:29.4
- 作者:Kinsella, Joseph M.;Ananda, Shalini;Andrew, Jennifer S.;Grondek, Joel F.;Chien, Miao-Ping;Scadeng, Miriam;Gianneschi, Nathan C.;Ruoslahti, Erkki;Sailor, Michael J.
- 通讯作者:Sailor, Michael J.
X-ray computed tomography imaging of breast cancer by using targeted peptide-labeled bismuth sulfide nanoparticles.
- DOI:10.1002/anie.201104507
- 发表时间:2011-12-16
- 期刊:
- 影响因子:16.6
- 作者:Kinsella, Joseph M.;Jimenez, Rebecca E.;Karmali, Priya P.;Rush, Anthony M.;Kotamraju, V. Ramana;Gianneschi, Nathan C.;Ruoslahti, Erkki;Stupack, Dwayne;Sailor, Michael J.
- 通讯作者:Sailor, Michael J.
Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein I-domain but a distinct tailspike receptor-binding domain.
噬菌体 CUS-3 病毒粒子的三维重建揭示了保守的外壳蛋白 I 结构域和独特的尾刺受体结合结构域。
- DOI:10.1016/j.virol.2014.06.017
- 发表时间:2014
- 期刊:
- 影响因子:3.7
- 作者:Parent,KristinN;Tang,Jinghua;Cardone,Giovanni;Gilcrease,EddieB;Janssen,MandyE;Olson,NormanH;Casjens,SherwoodR;Baker,TimothyS
- 通讯作者:Baker,TimothyS
Self-assembly of coherently dynamic, auxetic, two-dimensional protein crystals.
- DOI:10.1038/nature17633
- 发表时间:2016-05-19
- 期刊:
- 影响因子:64.8
- 作者:Suzuki Y;Cardone G;Restrepo D;Zavattieri PD;Baker TS;Tezcan FA
- 通讯作者:Tezcan FA
A morphology-dependent bio-organic template for inorganic nanowire synthesis.
- DOI:10.1002/smll.201101014
- 发表时间:2011-07
- 期刊:
- 影响因子:13.3
- 作者:Miao-Ping Chien;N. Gianneschi
- 通讯作者:Miao-Ping Chien;N. Gianneschi
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Timothy S Baker其他文献
Timothy S Baker的其他文献
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{{ truncateString('Timothy S Baker', 18)}}的其他基金
Parallel Software for Fast, Automated Determination of Virus Structures
用于快速、自动确定病毒结构的并行软件
- 批准号:
8277895 - 财政年份:2010
- 资助金额:
$ 200万 - 项目类别:
Parallel Software for Fast, Automated Determination of Virus Structures
用于快速、自动确定病毒结构的并行软件
- 批准号:
7785214 - 财政年份:2010
- 资助金额:
$ 200万 - 项目类别:
Parallel Software for Fast, Automated Determination of Virus Structures
用于快速、自动确定病毒结构的并行软件
- 批准号:
8075461 - 财政年份:2010
- 资助金额:
$ 200万 - 项目类别:
Parallel Software for Fast, Automated Determination of Virus Structures
用于快速、自动确定病毒结构的并行软件
- 批准号:
8475618 - 财政年份:2010
- 资助金额:
$ 200万 - 项目类别:
Parallel Software for Fast, Automated Determination of Virus Structures
用于快速、自动确定病毒结构的并行软件
- 批准号:
7820120 - 财政年份:2009
- 资助金额:
$ 200万 - 项目类别:
TRANSMISSION ELECTRON MICROSCOPE: STRUCTURAL BIOL: VIRUSES
透射电子显微镜:结构生物学:病毒
- 批准号:
6973809 - 财政年份:2004
- 资助金额:
$ 200万 - 项目类别:
TRANSMISSION ELECTRON MICROSCOPE: STRUCTURAL & CELL BIOL: MEMBRANE TRANSPORT, GA
透射电子显微镜:结构
- 批准号:
6973811 - 财政年份:2004
- 资助金额:
$ 200万 - 项目类别:
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