One-Photon/Two-Photon/Lifetime Confocal Microscope

一光子/双光子/终身共焦显微镜

• 批准号: 6732221
• 负责人: SHIMON WEISS
• 金额: $ 49.36万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732221
• 关键词: biomedical equipment purchase; confocal scanning microscopy; conformation; fluorescence microscopy

DESCRIPTION (provided by applicant): Understanding the properties of biological and artificial systems on the nanometer scale requires not only the ability to manipulate, fabricate, synthesize and assemble them with precise atomic/molecular level control, but also the ability to characterize and probe them with state-of-the-art nanoscale spectroscopic and imaging tools. Fluorescence microscopy offers many advantages for probing nanoscale systems. It is non-invasive and provides imaging in three dimensions; it has high sensitivity (down to the single molecule level), and allows the observation of molecular- and organelle-specific signals. We propose the acquisition of a combined One-Photon / Two-Photon / Lifetime Confo(;al Laser Scanning Microscope (1P/2P/Lifetime CLSM) to support research in artificial and biological nanoscale system assemblies conducted at the California Nano-Systems Institute (CNSI) at UCLA. The combined 1P/2P/Lifetime CLSM will allow the characterization of biological and artificial systems with the highest spatial and temporal resolutions achievable. This advanced optical tool would be particularly useful for: (1) The study dynamic interactions of biological macromolecules in living cells; (2) The visualization of multiple fluorescent markers at high resolution in a single optical plane (optical plane sectioning) and reconstruction of 3D images from consecutive sections; (3) The visualization and 3D sectioning of multiple fluorescent markers embedded in scattering media and tissues with high signal to noise and minimal photo-bleaching. The 1P/2P/Lifetime CLSM will be use to address outstanding questions covering a broad spectrum of topics in Chemistry, Biology, Physiology and Material Sciences. We propose to use the advanced performances of the 1P/2P/Lifetime CLSM in the following research projects: (1) The targeting of semiconductor nanocrystal probes in live cells, (2) The detection of conformational changes in voltage dependent ion channels and (3) The detection of structural and functional abnormalities in the transverse tubular system of normal and dystrophic muscle fibers. The acquisition of the 1P/2P/Lifetime CLSM would considerably enhance the nanoscale science performed at the CNSI and our understanding of the central functions of the cell.

描述（由申请人提供）：了解生物和人工系统在纳米尺度上的特性，不仅需要能够用精确的原子/分子水平控制来操纵、制造、合成和组装它们，还需要能够用最先进的纳米尺度光谱和成像工具来表征和探测它们。荧光显微镜为探测纳米级系统提供了许多优点。它是非侵入性的，提供三维成像；它具有高灵敏度（低至单分子水平），并允许观察分子和细胞器特异性信号。我们提议收购一个组合的单光子/双光子/寿命激光扫描显微镜（1P/2P/寿命CLSM），以支持在加州大学洛杉矶分校的加州纳米系统研究所（CNSI）进行的人工和生物纳米级系统组件的研究。组合的1P/2P/寿命CLSM将以最高的空间和时间分辨率表征生物和人工系统。这种先进的光学工具将特别有助于：(1)研究活细胞中生物大分子的动态相互作用；(2)多个荧光标记在单一光学平面上的高分辨率可视化（光学平面切片）和连续切片的三维图像重建；(3)嵌入散射介质和组织中的多个荧光标记的可视化和三维切片，具有高信噪比和最小光漂白。