BIOPHILE-80C Storage Unit with Individual Vial Retriever

BIOPHILE-80C 存储单元，带独立样品瓶回收器

• 批准号: 6732444
• 负责人: JOSHUA LABAER
• 金额: $ 40.21万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732444
• 关键词: biomedical automation; biomedical equipment purchase; cell bank /registry; clone cells; robotics

DESCRIPTION (provided by applicant): Biological research is shifting paradigms from molecular reductionist approaches to include larger-scale investigations of the entire proteome or genome. High-throughput (HT) biology began in earnest with the Human Genome Project; increasingly HT tools are being exploited for protein research. Given the importance of proteins in disease etiology and treatment, a major challenge facing biology is the elucidation of the physiological role of every protein. Using HT approaches, functional proteomics will address this challenge by expressing proteins at a scale of thousands at a time, and by subsequently assaying their functional properties, such as subcellular location, interacting partners, biochemical activity and regulated modification. A prerequisite for all these studies is a comprehensive collection of full-length expression-ready (FLEX) clones. Such a collection is being built using recombinational cloning, which allows rapid transfer into any vector, in frame and without mutation. These FLEXGene clones provide researchers with a powerful resource that expresses proteins in all experimental formats and at any chosen scale. As a complement to the FLEX repository, a collection is being assembled of clones encoding small inhibitory RNAs (siRNAs), which specifically inhibit the expression of their cognate gene. Together with FLEXGene, these collections allow users to up- or down-regulate specific proteins to study their function in biology and disease. Furthermore, assaying sets of hundreds of clones enables investigators to conduct large-scale screens to identify key proteins or pathways. The overall objective of this project is to build a robust repository that makes these clones readily available to researchers. However, the number of collected clones (already >15,000) exceeds the ability to distribute, track and retrieve them manually. User-requested sets of 200 clones can be dispersed over 100 different plates. Without automation, the fulfillment of dozens of clone requests is untenable on a just-in-time basis. This application requests funds to obtain an automated storage unit. The BIOPHILE storage unit with individual vial retriever (IVR) uniquely combines the reliability of robotically controlled retrieval with the required clone stability of -80 x C. Bar-coded samples are robotically retrieved without opening a freezer door, thus maintaining a stable temperature. While manual distribution accommodates fewer than 100 clones per day, the BIOPHILE has a capacity of 1,200 clones per day.

描述（申请人提供）：生物学研究正在从分子还原论方法转向包括整个蛋白质组或基因组的大规模研究。高通量（HT）生物学始于人类基因组计划，越来越多的HT工具被用于蛋白质研究。鉴于蛋白质在疾病病因学和治疗中的重要性，生物学面临的主要挑战是阐明每种蛋白质的生理作用。使用HT方法，功能蛋白质组学将通过一次表达数千种蛋白质，并随后测定其功能特性，如亚细胞位置，相互作用伴侣，生化活性和调节修饰来解决这一挑战。所有这些研究的先决条件是全面收集全长表达就绪（FLEX）克隆。目前正在利用重组克隆技术建立这样一个集合，这种技术允许在框内快速转移到任何载体中，而不发生突变。这些FLEXGene克隆为研究人员提供了一个强大的资源，可以以任何实验形式和任何选定的规模表达蛋白质。 作为FLEX储存库的补充，正在收集编码小抑制性RNA（siRNA）的克隆，这些小抑制性RNA特异性抑制其同源基因的表达。与FLEXGene一起，这些集合允许用户上调或下调特定蛋白质，以研究它们在生物学和疾病中的功能。此外，分析数百个克隆使研究人员能够进行大规模筛选以识别关键蛋白质或途径。该项目的总体目标是建立一个强大的知识库，使这些克隆随时可供研究人员使用。 但是，收集的克隆数量（已经超过15，000）超过了手动分发、跟踪和检索它们的能力。用户要求的200个克隆集可以分散在100个不同的平板上。如果没有自动化，就无法及时满足数十个克隆请求。这项申请要求提供资金，以获得一个自动储存单元。带有单个小瓶回收器（IVR）的BIOPHILE储存装置独特地将机器人控制回收的可靠性与所需的-80 x C克隆稳定性相结合。条形码样品在不打开冷冻室门的情况下自动取出，从而保持稳定的温度。人工分发每天只能容纳不到100个克隆，而BIOPHILE每天可容纳1，200个克隆。