Bioimaging Facility Confocal Microscope

生物成像设备共焦显微镜

• 批准号: 6734880
• 负责人: JAMES H KEEN
• 金额: $ 36.14万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6734880
• 关键词: biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): This application is for support to purchase a single photon Zeiss 510 Meta laser confocal microscope. The instrument will be housed within the Bioimaging Facility of the Kimmel Cancer Center of Thomas Jefferson University. This Facility provides light microscopy services to users within the Kimmel Cancer Center, throughout Thomas Jefferson University, and as needed and available to investigators at neighboring research institutions. This instrument is critically needed to replace the existing Bio-Rad MRC-600 confocal microscope, which is over 11 years old, limited in its capabilities and increasingly problematic to maintain. Importantly, the requested instrument will provide new capabilities not currently available. These include the ability to image three distinct signals with precise registration and with high sensitivity, to conduct quantitative FRAP experiments on user-defined areas, to reliably reject signal bleed through, to carry out FRET experiments, and to provide the flexibility to resolve overlapping emission spectra. This application is submitted by a group of major users at Thomas Jefferson University within the Kimmel Cancer Center, in the Farber Institute of Neuroscience, and in the Department of Neurology, all of whom are well funded with multiple NIH-supported projects involving confocal microscopy, and extensive experience and need for confocal microscopy in their work. In addition, a group of new users includes investigators at earlier stages of career with particular need for confocal microscopy that cannot readily be addressed by the existing instrumentation, such as imaging of Zebrafish and immune synapse formation. Acquisition of the proposed instrument will allow these and other investigators to more effectively carry out their work, and to use and develop state-of-the-art techniques in confocal microscopy in their studies.

说明（由申请人提供）：本申请是为了支持购买单光子蔡司510 Meta激光共聚焦显微镜。该仪器将被安置在托马斯杰斐逊大学Kimmel癌症中心的生物成像设施内。该设施为Kimmel癌症中心内的用户提供光学显微镜服务，整个托马斯杰斐逊大学，并根据需要提供给邻近研究机构的研究人员。现有的Bio-Rad MRC-600共聚焦显微镜已经使用了11年以上，功能有限，维护起来也越来越困难。重要的是，所要求的仪器将提供目前不具备的新功能。其中包括能够以精确的配准和高灵敏度对三种不同的信号进行成像，在用户定义的区域进行定量FRAP实验，可靠地拒绝信号溢出，进行FRET实验，并提供解决重叠发射光谱的灵活性。