Pyrosequencer

焦磷酸测序仪

• 批准号: 6730883
• 负责人: Isaac I Wirgin
• 金额: $ 12.01万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730883
• 关键词: biomedical equipment purchase; genotype; high throughput technology; nucleic acid sequence; polymerase chain reaction; single nucleotide polymorphism

DESCRIPTION (provided by applicant): Efforts to evaluate the relationship between genetic polymorphisms and susceptibilities to various types of cancers have intensified in recent years in the Department of Environmental Medicine and have resulted in successful collaborative NIH grant applications between investigators in the Epidemiology and Molecular Toxicology/Carcinogenesis Programs in the Department. Additionally, several members of the Department have long NIH funded track records in evaluating the epigenetic effects of DNA methylation at CpG islands in carcinogenesis models. Furthermore, increased efforts in these areas are reflected in Environmental Medicine's successful acquisition of a Superfund Basic Research Program Center Grant entitled "Genetic/Epigenetic Susceptibility to Superfund Chemicals." Other NIH funded projects by Department investigators involve the development of linkage maps with single nucleotide polymorphisms as markers to identify novel genes associated with toxicant induced disease. With this application, we propose to acquire a Pyrosequencer TM to be housed in the shared Molecular Facility Core of Environmental Medicine's longstanding NIEHS Center. This instrument is needed to increase the throughput and accuracy of genotyping for the multiple NIH funded genotyping studies by members of this Department. By increasing sample and loci number, these investigations will be much more sensitive in evaluating the effects of genetic polymorphisms on susceptibility to disease. Similarly, investigators studying the relationships between methylation status and carcinogenesis will be able to expand their analyses to include multiple CpG islands within a short stretch of DNA and will now be able to quantitatively estimate the percentage of hypermethylated nucleotides at individual islands. The NIH grant support obtained by the several research groups needing this instrument shows that they are conducting high-quality science; with the acquisition of the Pyrosequencer they will be able to perform more extensive and detailed investigations that will enable them to stay at the forefront in their research areas.

描述（由申请人提供）：近年来，环境医学系加强了对遗传多态性与各种癌症易感性之间关系的评估，并导致流行病学和分子毒理学/致癌计划研究人员之间成功合作NIH资助申请。此外，该部门的几名成员在评估致癌模型中CpG岛DNA甲基化的表观遗传效应方面有着长期的NIH资助记录。此外，环境医学在这些领域加大了努力，这反映在环境医学成功获得了超级基金基础研究方案中心赠款，题为“对超级基金化学品的遗传/表观遗传易感性”。“其他由NIH资助的项目涉及开发以单核苷酸多态性为标记的连锁图，以识别与毒物诱导疾病相关的新基因。有了这个应用程序，我们建议获得一个Pyrosequencer TM被安置在共享的分子设施核心环境医学的长期NIEHS中心。该仪器是需要增加的通量和基因分型的准确性，为多个NIH资助的基因分型研究，由本部门的成员。通过增加样本和基因座数量，这些研究将在评估遗传多态性对疾病易感性的影响方面更加敏感。类似地，研究甲基化状态与致癌作用之间关系的研究人员将能够扩展他们的分析，以包括DNA短片段内的多个CpG岛，并且现在能够定量估计单个岛上高甲基化核苷酸的百分比。需要该仪器的几个研究小组获得了NIH的资助，这表明他们正在进行高质量的科学研究;随着Pyrosequencer的收购，他们将能够进行更广泛和详细的研究，使他们能够在研究领域保持领先地位。