Purchase of Confocal Laser Scanning Microscope

描述（由申请人提供）：要求购买一台激光扫描共聚焦显微镜，用于拉霍亚分子医学研究所的核心显微镜成像设备。虽然几个现代荧光显微镜可供一般使用的工作人员，我们没有共聚焦显微镜在这个网站。考虑到拉霍亚地区对其他共聚焦显微镜收费服务的高需求，以及许多样品（即活体成像、活细胞、新鲜组织标本）运输的时效性和难度，校园共聚焦显微镜的增加将极大地增强正在进行的nih资助的研究项目的多样性。例如，由于隔离和手术需要的困难，在校外无法对植入动物模型的活体腔室进行成像。正如本提案中详述的那样，我们已经成立了一个委员会（Brian Eliceiri、Carole Banka、Eugene Levin和Ingrid Schraufstatter），以确定主要用户的核心小组，确定研究所的具体需求，并选择适当的仪器来完成我们研究小组的目标。这种共聚焦显微镜的操作将集成到一个中央位置，开放访问的核心成像设施，该设施已建立用于体内成像以及时间过程细胞迁移和一般显微镜。

DESCRIPTION (provided by applicant): The purchase of a laser scanning confocal microscope is requested for the core microscope imaging facility at the La Jolla Institute for Molecular Medicine. While several modern fluorescent microscopes are available for general use by the staff, we do not have a confocal microscope at this site. Based upon the high demand placed on the fee-for-service access to other confocal microscopes in the La Jolla area and the time-sensitive nature and difficulty in transporting many samples (i.e. intravital imaging, live cells, fresh tissue specimens), the addition of an on-campus confocal microscope would greatly enhance a diverse array of ongoing NIH-funded research programs. For example, imaging of intravital chambers implanted in animal models is not feasible off-campus due to the difficulties of quarantine and surgical needs. As detailed in this proposal, we have formed a committee (Brian Eliceiri, Carole Banka, Eugene Levin, and Ingrid Schraufstatter) to identify a core group of Primary Users, determined the specific needs of the institute, and chosen appropriate instrumentation to accomplish the goals of our research group. The operation of this confocal microscope will be integrated into a centrally located, open access core imaging facility that has been established for in vivo imaging as well as time course cell migration and general microscopy. Following extensive consultation and analysis of various competing confocal microscope systems, we have identified the Olympus Fluoview 500 as the ideal system for a core environment, maximizing ease of use with capable specifications for current and future requirements. Six of the seven Primary Users have active R0l-funded research programs through the NIH, with the seventh investigator supported by other sources and an RO1 submitted and pending review. The cost of the requested confocal microscope is $274,579 and will be matched by an institutional commitment for an annual $10,000 serve contract, the full cost of future changes/upgrades, and 80% salary support for a technician to manage the microscope. The balance of the salary will come from a cost-recapture from individual grants, based on usage. In combination with our existing imaging infrastructure, the proposed confocal microscope will be an important addition to maximize the impact of our existing research programs.