IVIS Spectrum Imaging System

描述(由申请人提供)：请为位于Hillcrest的加州大学圣地亚哥医学中心(UCSDMC)的核心成像设施购买生物发光成像(BLI)系统。虽然医学中心为特定的用户群体提供了各种显微镜和非侵入性成像仪(即磁共振成像)，但我们在该网站没有BLI系统。由于加州大学圣迭戈分校唯一的其他BLI成像仪对收费服务的要求很高(即，它位于拉霍亚)，运输许多样本(即活体成像、活细胞、新鲜组织标本)的难度很大，因此UCSDMC增加一个核心BLI系统将极大地增强一系列正在进行的NIH资助的研究项目。例如，由于检疫和手术需要的困难，在校园外对植入动物模型的活体房间进行成像是不可行的。如本建议书所述，我们成立了一个委员会(Brian Eliceiri、Tony Yaksh、Andrew Baird和Allen Ryan)，以确定主要用户的核心群体，确定UCSDMC的特定需求，并选择适当的工具来实现我们研究小组的目标。该BLI系统的运行将被整合到一个位于中心的开放式核心成像设施中，该设施已经建立，用于活体成像以及时间进程研究和一般成像。在对各种竞争的BLI系统进行了广泛的咨询和分析后，我们确定了卡利珀生命科学IVIS Spectrum是核心环境的理想系统，最大限度地提高了易用性，具有满足当前和未来需求的强大规格。所有主要和次要用户都有NIH通过NIH资助的活跃研究项目，其中一名调查人员是P20探索中心的PI。所要求的BLI系统的费用为364,672美元，并将由机构承诺提供50%的工资支持，以支持一名技术人员管理该系统和安全的、中央可进入的空间。工资的余额将来自根据使用情况从个人补助金中重新获得的成本。与我们现有的成像基础设施相结合，拟议的BLI系统将是一个重要的补充，以最大限度地发挥我们现有研究计划的影响。 公共卫生相关性：拨款提案是为加州大学圣地亚哥分校医疗中心(UCSDMC)的核心成像设施申请购买生物发光成像(BLI)系统，我们在那里没有BLI系统。这项提议的目标是将其能力整合到我们现有的成像基础设施中，并最大限度地发挥我们现有NIH资助的研究项目的影响。

DESCRIPTION (provided by applicant): The purchase of a bioluminescent imaging (BLI) system is requested for the core imaging facility at the University of California San Diego Medical Center (UCSDMC), Hillcrest. While various microscopes, and non-invasive imagers (i.e. magnetic resonance imaging) are available at the Medical Center for select user groups, we do not have a BLI system at this site. Based upon the high demand placed on the fee-for- service access (i.e. it is located in La Jolla) to the only other BLI imager at UCSD, difficulty in transporting many samples (i.e. intravital imaging, live cells, fresh tissue specimens), the addition of a core BLI system at the UCSDMC would greatly enhance a diverse array of ongoing NIH-funded research programs. For example, imaging of intravital chambers implanted in animal models is not feasible off-campus due to the difficulties of quarantine and surgical needs. As detailed in this proposal, we have formed a committee (Brian Eliceiri, Tony Yaksh, Andrew Baird, and Allen Ryan) to identify a core group of Primary Users, determined the specific needs of the UCSDMC, and chosen appropriate instrumentation to accomplish the goals of our research group. The operation of this BLI system will be integrated into a centrally located, open access core imaging facility that has been established for in vivo imaging as well as time course studies and general imaging. Following extensive consultation and analysis of various competing BLI systems, we have identified the Caliper Life Sciences IVIS Spectrum as the ideal system for a core environment, maximizing ease of use with capable specifications for current and future requirements. All Primary and Secondary Users have active NIH-funded research programs through the NIH, with one of the investigators the PI of a P20 Exploratory Center. The cost of the requested BLI system is $364,672 and will be matched by an institutional commitment for 50% salary support for a technician to manage the system and secured, centrally-accessible space. The balance of the salary will come from a cost-recapture from individual grants, based on usage. In combination with our existing imaging infrastructure, the proposed BLI system will be an important addition to maximize the impact of our existing research programs. PUBLIC HEALTH RELEVANCE: The grant proposal is a request for the purchase of a bioluminescent imaging (BLI) system is requested for the core imaging facility at the University of California San Diego Medical Center (UCSDMC), Hillcrest, where we do not have a BLI system. The objective of this proposal is to integrate its capabilities into our existing imaging infrastructure and maximize the impact of our existing NIH-funded research programs.