NCRR: Purchase of Zeiss LSM5 Pascal Confocal Microscope

NCRR：购买蔡司 LSM5 帕斯卡共焦显微镜

• 批准号: 6730878
• 负责人: Karen M Ottemann
• 金额: $ 25.81万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730878
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a laser scanning confocal microscope for a group of NIH-funded investigators from several departments at the University of California at Santa Cruz. The requested system consists of a Zeiss LSM5 Confocal Microscope System, with the addition of a Bioptechs FCS2 Stage/Chamber Heating System and Objective Heater. This microscope system would be part of a Biomedical Core Facility, and as such, open to all NIH-funded investigators. Having such a microscope would greatly enhance and support the top-notch research at UCSC because currently, although there are two confocal microscopes on campus, both are privately held and thus access is extremely limited. The LSM5 Pascal is an outstanding choice for a core facility due to its ease of use, speed of scanning and optical resolution. The major user group seeks to bring the power of confocal microscopy to studies of neuronal receptor cellular localization, neuronal targeting, Vibrio cholerae biofilm formation, bacterial movement within animal organs, and immune cell interactions during immunological tolerance. Several of the major users have experience with confocal microscopy, including use of the LSM5 Pascal, and there is no question of its utility with regards to our technical needs. The confocal will be housed within the Department of Environmental Toxicology, and supported by the Division of Physical and Biological Sciences in conjunction with the major users group. The possibility of a shared laser scanning confocal imaging system at UC Santa Cruz is timely because of current efforts to expand Biomedical Sciences; thus this equipment will both enhance research and provide training for graduate students and post-doctoral research fellows.

描述（由申请人提供）：本提案要求资金为圣克鲁斯的加州大学几个系的NIH资助的研究人员购买激光扫描共聚焦显微镜。申请的系统包括Zeiss LSM 5共聚焦显微镜系统，以及Bioptechs FCS 2载物台/腔室加热系统和物镜加热器。这个显微镜系统将是生物医学核心设施的一部分，因此，对所有NIH资助的研究人员开放。拥有这样的显微镜将大大提高和支持在UCSC一流的研究，因为目前，虽然有两个共焦显微镜在校园，都是私人持有，因此访问是非常有限的。LSM 5 Pascal是核心设施的杰出选择，因为它易于使用，扫描速度和光学分辨率。主要用户组旨在将共聚焦显微镜的力量用于神经元受体细胞定位，神经元靶向，霍乱弧菌生物膜形成，动物器官内的细菌运动以及免疫耐受期间免疫细胞相互作用的研究。几个主要用户都有使用共聚焦显微镜的经验，包括使用LSM 5 Pascal，并且毫无疑问，它的实用性符合我们的技术需求。共焦系统将设在环境毒理学系内，并由物理和生物科学司与主要用户组共同提供支持。在加州大学圣克鲁斯共享激光扫描共聚焦成像系统的可能性是及时的，因为目前的努力，以扩大生物医学科学;因此，这种设备将既加强研究，并为研究生和博士后研究员提供培训。