NCRR: Purchase of Zeiss LSM5 Pascal Confocal Microscope

描述（由申请人提供）：本申请申请为一组由美国国立卫生研究院资助的来自加州大学圣克鲁斯分校几个系的研究人员购买激光扫描共聚焦显微镜。所要求的系统包括一个蔡司LSM5共聚焦显微镜系统，加上一个Bioptechs FCS2级/室加热系统和物镜加热器。该显微镜系统将成为生物医学核心设施的一部分，因此，对所有nih资助的研究人员开放。拥有这样一台显微镜将极大地加强和支持UCSC的顶尖研究，因为目前，虽然校园里有两台共聚焦显微镜，但都是私人拥有的，因此使用极为有限。LSM5 Pascal是核心设施的杰出选择，因为它易于使用，扫描速度和光学分辨率。主要用户群体寻求将共聚焦显微镜的力量引入神经元受体细胞定位，神经元靶向，霍乱弧菌生物膜形成，动物器官内细菌运动以及免疫耐受期间免疫细胞相互作用的研究中。几个主要用户都有使用共聚焦显微镜的经验，包括使用LSM5 Pascal，毫无疑问，它对我们的技术需求是有用的。该中心将设在环境毒理学部，并由物理和生物科学司与主要用户组一起提供支助。加州大学圣克鲁斯分校共享激光扫描共聚焦成像系统的可能性是及时的，因为目前正在努力扩大生物医学科学；因此，该设备既可以加强研究，又可以为研究生和博士后研究员提供培训。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a laser scanning confocal microscope for a group of NIH-funded investigators from several departments at the University of California at Santa Cruz. The requested system consists of a Zeiss LSM5 Confocal Microscope System, with the addition of a Bioptechs FCS2 Stage/Chamber Heating System and Objective Heater. This microscope system would be part of a Biomedical Core Facility, and as such, open to all NIH-funded investigators. Having such a microscope would greatly enhance and support the top-notch research at UCSC because currently, although there are two confocal microscopes on campus, both are privately held and thus access is extremely limited. The LSM5 Pascal is an outstanding choice for a core facility due to its ease of use, speed of scanning and optical resolution. The major user group seeks to bring the power of confocal microscopy to studies of neuronal receptor cellular localization, neuronal targeting, Vibrio cholerae biofilm formation, bacterial movement within animal organs, and immune cell interactions during immunological tolerance. Several of the major users have experience with confocal microscopy, including use of the LSM5 Pascal, and there is no question of its utility with regards to our technical needs. The confocal will be housed within the Department of Environmental Toxicology, and supported by the Division of Physical and Biological Sciences in conjunction with the major users group. The possibility of a shared laser scanning confocal imaging system at UC Santa Cruz is timely because of current efforts to expand Biomedical Sciences; thus this equipment will both enhance research and provide training for graduate students and post-doctoral research fellows.