Enhancing motor protein performance for nanopore DNA sequencing via directed and Darwinian evolutionary approaches

该项目的目标是鉴定和开发能够作为纳米孔测序的高性能运动蛋白的DNA解旋酶。将采用两种方法。第一种方法将涉及从各种各样的物种中生产未经测试的解旋酶蛋白，以确定具有有利操作特性的目标（达尔文方法）。第二步是建立一个体内方案来生成和筛选我们所建立的解旋酶靶点的大量序列变体（定向方法）。这两种方法的有希望的命中将受到使用一系列生化，生物物理和结构技术的详细表征。

The goal of this project is to identify and develop DNA helicases that are capable of very high performance as motor proteins for nanopore sequencing. Two approaches will be used. The first will involve producing untested helicase proteins from a diverse range of species to identify targets with favourable operating properties (the Darwinian approach). The second will establish an in vivo protocol to generate and screen large numbers of sequence variants of our established helicase targets (the directed approach). Promising hits from both approaches will be subjected to detailed characterisation using a range of biochemical, biophysical and structural techniques.