Enhancing motor protein performance for nanopore DNA sequencing via directed and Darwinian evolutionary approaches

通过定向进化和达尔文进化方法增强纳米孔 DNA 测序的运动蛋白性能

• 批准号: 2607264
• 金额: --
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2607264
• 关键词: Enhancing; motor; protein; performance; nanopore

The goal of this project is to identify and develop DNA helicases that are capable of very high performance as motor proteins for nanopore sequencing. Two approaches will be used. The first will involve producing untested helicase proteins from a diverse range of species to identify targets with favourable operating properties (the Darwinian approach). The second will establish an in vivo protocol to generate and screen large numbers of sequence variants of our established helicase targets (the directed approach). Promising hits from both approaches will be subjected to detailed characterisation using a range of biochemical, biophysical and structural techniques.

该项目的目标是鉴定和开发能够作为用于纳米孔测序的马达蛋白的非常高性能的DNA解旋酶。将采用两种方法。第一个将涉及从各种物种中生产未经测试的解旋酶蛋白，以确定具有有利操作特性的目标（达尔文方法）。第二个将建立一个体内方案，以产生和筛选我们建立的解旋酶靶点的大量序列变体（定向方法）。这两种方法的有希望的命中将受到详细的表征使用一系列的生物化学，生物物理和结构技术。