NELL-1, A Cbfa1 DOWNSTREAM TARGET, IN BONE FORMATION

NELL-1,Cbfa1 下游靶标,在骨形成中

基本信息

  • 批准号:
    7071662
  • 负责人:
  • 金额:
    $ 33.95万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-07-01 至 2009-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Cbfa1 mediates mesenchymal cell commitment to an osteochondroprogenitor lineage and supports continued differentiation and function in committed osteoblasts. However, definitive downstream mediators of Cbfa1 that critically impact osteoblast differentiation and bone formation have yet to be described. Ne1 (a protein strongly expressed in neural tissue encoding epidermal growth factor like domain) was first identified in chicken embryos. The preliminary data shows that Nell-1 (Ne1-like molecule-1) in vivo is associated with normal and pathological suture fusion, while Nell- 1 in vitro is associated with increased osteoblast differentiation and mineralization. Furthermore, the human Nell-1 promoter contains three osteoblast-specific cis-acting element 2 (OSE2) response elements for Cbfa1 and that Nell-1 overexpression mice can functionally compensate for some aspects of intramembranous bone deficiency in heterozygous Cbfa1 deficient mice. These data have led to the hypothesis that Nell-1 is a central downstream target of Cbfa1 that supports continued differentiation and function in committed osteoblasts and proper Nell-1 expression is required for normal membranous bone growth and formation. To test this hypothesis, this proposal will analyze the following: 1) the regulation of Nell-1 transcription by Cbfa1 during osteoblast formation and function and by Cbfa1 through OSE2 response elements; 2) the functional compensation of Cbfa1 deficiency by Nell-1 in vivo and in vitro; and 3) the effects of targeted Nell-1 disruption in vivo. If Nell-1 is proven to be a critical mediator of intramembranous bone formation, it can further aid in understanding the development of intramembranous vs. endochondral bone. In addition, since sutures are major sites of calvarial intramembranous bone growth the close association of Nell-1 with calvarial sutures can also impact present knowledge on cranial vault development. Lastly, Nell-1 can be tremendously useful as a clinical tool to inhibit or induce intramembranous bone growth, with the secretory nature of Nell-1 making this utility even more feasible. Controlled inhibition of intramembranous bone growth by anti-Nell-1 strategies in CS patients may offer a less invasive, biologically based, therapeutic alternative to radical surgical craniofacial reconstruction. In addition, the ability of Nell- 1 to induce calvarial osteoblast differentiation may be clinically applied to situations where membranous bone formation and regeneration are desirable (e.g., cleft lip or cleft palate repair and craniofacial distraction osteogenesis).
描述(由申请人提供):Cbfa 1介导间充质细胞定向为骨软骨祖细胞谱系,并支持定向成骨细胞的持续分化和功能。然而,决定性的下游介质的Cbfa 1,严重影响成骨细胞分化和骨形成尚未被描述。 Ne 1(在神经组织中强烈表达的编码表皮生长因子样结构域的蛋白)首先在鸡胚中被鉴定。初步数据显示,体内Nell-1(Ne 1样分子-1)与正常和病理性缝线融合相关,而体外Nell- 1与成骨细胞分化和矿化增加相关。此外,人Nell-1启动子含有三个成骨细胞特异性顺式作用元件2(OSE 2)的Cbfa 1的反应元件,Nell-1过表达小鼠可以在功能上弥补杂合Cbfa 1缺陷小鼠膜内骨缺损的某些方面。这些数据导致了这样的假设,即Nell-1是Cbfa 1的中心下游靶点,其支持定向成骨细胞的持续分化和功能,并且正常的膜骨生长和形成需要适当的Nell-1表达。为了验证这一假设,本提案将分析以下内容:1)在成骨细胞形成和功能期间Cbfa 1以及Cbfa 1通过OSE 2反应元件对Nell-1转录的调节; 2)Nell-1在体内和体外对Cbfa 1缺陷的功能补偿;以及3)靶向Nell-1破坏在体内的影响。 如果Nell-1被证明是膜内骨形成的关键介质,它可以进一步帮助理解膜内骨与软骨内骨的发育。此外,由于缝是颅骨膜内骨生长的主要部位,Nell-1与颅骨缝的密切联系也可能影响目前对颅穹窿发育的认识。最后,Nell-1可以作为抑制或诱导膜内骨生长的临床工具非常有用,Nell-1的分泌性质使得这种用途更加可行。通过抗Nell-1策略控制CS患者膜内骨生长的抑制可能为根治性手术颅面重建提供一种侵入性较小、基于生物学的治疗替代方案。此外,Nell- 1诱导颅骨成骨细胞分化的能力可在临床上应用于需要膜性骨形成和再生的情况(例如,唇裂或腭裂修复和颅面牵引成骨)。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KANG TING其他文献

KANG TING的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KANG TING', 18)}}的其他基金

Novel peptide-impregnated hydrogel as a wound healing device
新型肽浸渍水凝胶作为伤口愈合装置
  • 批准号:
    10156930
  • 财政年份:
    2016
  • 资助金额:
    $ 33.95万
  • 项目类别:
NELL-1, A Cbfa1 DOWNSTREAM TARGET, IN BONE FORMATION
NELL-1,Cbfa1 下游靶标,在骨形成中
  • 批准号:
    6899379
  • 财政年份:
    2004
  • 资助金额:
    $ 33.95万
  • 项目类别:
NELL-1, A Cbfa1 DOWNSTREAM TARGET, IN BONE FORMATION
NELL-1,Cbfa1 下游靶标,在骨形成中
  • 批准号:
    7253251
  • 财政年份:
    2004
  • 资助金额:
    $ 33.95万
  • 项目类别:
NELL-1, A Cbfa1 DOWNSTREAM TARGET, IN BONE FORMATION
NELL-1,Cbfa1 下游靶标,在骨形成中
  • 批准号:
    6813346
  • 财政年份:
    2004
  • 资助金额:
    $ 33.95万
  • 项目类别:
Nell-1, A Cbfa 1 Downstream Target, In Bone Formation
Nell-1,Cbfa 1 下游目标,在骨形成中
  • 批准号:
    7839166
  • 财政年份:
    2004
  • 资助金额:
    $ 33.95万
  • 项目类别:
NELL-1, A Cbfa1 DOWNSTREAM TARGET, IN BONE FORMATION
NELL-1,Cbfa1 下游靶标,在骨形成中
  • 批准号:
    7456445
  • 财政年份:
    2004
  • 资助金额:
    $ 33.95万
  • 项目类别:
Mechanistic Role of NELL-1 in Premature Suture Closure
NELL-1 在缝线过早闭合中的机制作用
  • 批准号:
    6485869
  • 财政年份:
    2002
  • 资助金额:
    $ 33.95万
  • 项目类别:
Mechanistic Role of NELL-1 in Premature Suture Closure
NELL-1 在缝线过早闭合中的机制作用
  • 批准号:
    6651152
  • 财政年份:
    2002
  • 资助金额:
    $ 33.95万
  • 项目类别:
NEL2 GENE IN BONE FORMATION AND CRANIAL SUTURE CLOSURES
NEL2 基因在骨形成和颅缝闭合中的作用
  • 批准号:
    2829902
  • 财政年份:
    1999
  • 资助金额:
    $ 33.95万
  • 项目类别:
NEL-2 GENE IN BONE FORMATION & CRANIAL SUTURE CLOSURES
NEL-2 基因在骨形成中的作用
  • 批准号:
    6176897
  • 财政年份:
    1999
  • 资助金额:
    $ 33.95万
  • 项目类别:

相似海外基金

Hedgehog signalling in T-cell differentiation and function
T 细胞分化和功能中的 Hedgehog 信号传导
  • 批准号:
    BB/Y003454/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.95万
  • 项目类别:
    Research Grant
Comparative single-cell analysis of disease-derived stem cells to identify the cell fate defect on the cell differentiation trajectory
对疾病来源的干细胞进行比较单细胞分析,以确定细胞分化轨迹上的细胞命运缺陷
  • 批准号:
    23H02466
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The role of cell differentiation in colorectal cancer progression
细胞分化在结直肠癌进展中的作用
  • 批准号:
    23K06661
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
TOX-driven CD8 T cell differentiation and dysfunction in tumors
TOX驱动的肿瘤中CD8 T细胞分化和功能障碍
  • 批准号:
    10586679
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
Dissecting the role of hypoxia in T cell differentiation in cancer
剖析缺氧在癌症 T 细胞分化中的作用
  • 批准号:
    10578000
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
Mechanisms mediating human enteroendocrine cell differentiation and function
介导人肠内分泌细胞分化和功能的机制
  • 批准号:
    10739834
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
New strategies in cell replacement therapies for diabetes: role of USP7 in iPSC and adult organoids beta cell differentiation
糖尿病细胞替代疗法的新策略:USP7 在 iPSC 和成体类器官 β 细胞分化中的作用
  • 批准号:
    MR/X01813X/1
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
    Research Grant
Elucidation of molecular mechanisms of immune cell differentiation of a novel Rab protein in hematopoietic stem cells
阐明造血干细胞中新型Rab蛋白免疫细胞分化的分子机制
  • 批准号:
    23K16122
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Role of alveolar fibroblasts in extracellular matrix organization and alveolar type 1 cell differentiation
肺泡成纤维细胞在细胞外基质组织和肺泡1型细胞分化中的作用
  • 批准号:
    10731854
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
Exhaustive Identification of Essential Genes for Human Taste Cell Differentiation ~Development of a Method for Inducing Differentiation of Taste Buds from ES/iPS Cells~
彻底鉴定人类味觉细胞分化必需基因~开发诱导ES/iPS细胞味蕾分化的方法~
  • 批准号:
    23K09214
  • 财政年份:
    2023
  • 资助金额:
    $ 33.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了