Molecular and Immunopathology of Experimental and Clinic

通过常规病理学、免疫组织化学、原位杂交、显微解剖和各种分子技术，包括PCR、RT-PCR和RFLP测定，分析患者标本中眼部炎症细胞、退行性细胞和肿瘤细胞及其产物的身份和地形位置。尖端现代技术的应用使我们能够对疾病进行准确的病理评估，指导我们为患者选择合适的治疗方法，并帮助我们更好地了解疾病机制。在2003财年，我们在研究中继续并完成了以下工作：眼部淋巴瘤细胞中基因和蛋白的检测：我们继续检测IgH基因重排和玻璃体IL-10升高，作为原发性眼内b细胞淋巴瘤（PIOL）诊断的有用辅助手段。玻璃体中高比例的IL-10和IL-6有利于PIOL的发生。1.0的临界值诊断PIOL的正确率为74.7%（敏感性74.3%，特异性75.0%）。2. 眼部炎症及其他疾病的新病理及发病机制：利用显微解剖和PCR技术，我们可以检测到眼部组织浆菌病综合征、眼眶淋巴瘤分子单克隆和肾移植后眼部淋巴细胞增生性疾病的荚膜组织浆体基因。我们是第一个报道Waldenstrom巨球蛋白血症相关的副肿瘤视网膜病变和年龄相关性黄斑变性中快速进展的纤维血管新血管膜的免疫病理的研究小组。3. 各种炎症性眼病的实验模型：我们继续研究炎症介质和细胞因子在内毒素诱导的葡萄膜炎（EIU）模型中的作用，EIU是人类前葡萄膜炎的模型。我们已经评估了性激素对实验性自身免疫性葡萄膜炎大鼠的影响。在与博士合作。Caspi和Gery等人发现并发表了几种新的眼部炎症模式和机制。

The identity and topographic location of ocular inflammatory, degenerative, and tumor cells and their products in patient specimens are analyzed by routine pathology, immunohistochemistry, in situ hybridization, microdissection and various molecular techniques including PCR, RT-PCR, and RFLP assay. The application of cutting-edge modern technology allows us to provide accurate pathological assessments of the disease, guides us in selecting an appropriate therapy for the patient, and helps us to better understand the disease mechanisms. In FY2003, we continued and accomplished the following in our research: 1. Detection of Genes and Proteins in Ocular Lymphoma Cells: We continued to detect IgH gene rearrangements and vitreal IL-10 elevations as useful adjuncts for primary intraocular B-cell lymphoma (PIOL) diagnosis. A high ratio of IL-10 to IL-6 in the vitreous was in favor of PIOL. A 1.0 cutoff rule for the ratio was correct 74.7% of the time (sensitivity 74.3%, specificity 75.0%) for the diagnosis of PIOL. 2. New Pathology and Pathogenesis of Ocular Inflammation and Other Diseases: Using microdissection and PCR, we are able to detect the Histoplasma capsulatum gene in ocular histoplasmosis syndrome, molecular monoclonality in orbital lymphoma and ocular post-transplant lymphoproliferative disorder after renal transplantation. We were the first group to report Waldenstrom macroglobulinemia-associated paraneoplastic retinopathy and the immunopathology of rapid progressive fibrovascular neovascular membrane in age-related macular degeneration. 3. Experimental Models for Various Inflammatory Ocular Diseases: We continued to investigate the role of inflammatory mediators and cytokines in the endotoxin-induced uveitis (EIU) model, a model for human anterior uveitis. We have already evaluated the effect of sex hormones on experimental autoimmune uveitis in rats. In collaboration with Drs. Caspi and Gery, several new modes and mechanisms of ocular inflammation have been discovered and published.