NE Modulation of Olfactory Bulb During Learning in Mice

描述(由申请人提供)：我们的目标是在主嗅球(MOB)中的去甲肾上腺素(NE)调制、MOB网络输出的变化和行为之间建立直接联系。我们假设学习将通过去甲肾上腺素诱导二尖瓣细胞团活动与习得气味的同步性的总体增加。我们有初步证据表明，完全阻断球茎中的去甲肾上腺素信号会损害成年小鼠辨别气味线索的能力，但不会阻碍它们的能力。我们目前正在通过记录参与嗅觉辨别任务的清醒行为小鼠的二尖瓣细胞层，即嗅球的输出神经元，来表征嗅球神经元网络中发生的调制。最后，我们将确定阻断NE信号是否会损害在行为过程中观察到的二尖瓣细胞活动的变化。

DESCRIPTION (provided by applicant): Our goal is to make a direct connection between noradrenaline (NE) modulation in the main olfactory bulb (MOB), changes in MOB network output, and behavior. We hypothesize that learning will induce, through NE, an overall increase in synchronization of mitral cell ensemble activity to the learned odor. We have preliminary evidence that complete blockade of NE signaling in the bulb impairs but does not impede an adult mouse's ability to discriminate between odor cues. We are currently characterizing the modulation occurring in olfactory bulb neuronal networks by recording from the mitral cell layer, the output neurons of the olfactory bulb, in awake-behaving mice engaged in an olfactory discrimination task. Finally, we will determine whether blockade of NE signaling will impair the changes in mitral cell activity observed during behavior.