Pregnanolone's effects in DBA/2J and C57BL/6J mice

描述（由申请方提供）：神经类固醇代表一类新型内源性化合物，能够发挥快速、非基因组效应并产生各种内分泌和行为效应。这些类固醇在乙醇的行为效应中的作用最近受到了广泛的关注。在药物辨别的行为测定中，神经类固醇如别孕烯醇酮和孕烯醇酮产生与乙醇非常相似的辨别刺激。神经类固醇的这些行为效应需要彻底检查，以了解这些内源性化合物在乙醇作用中的作用。药物识别通常用于识别药物行为效应的潜在受体机制。本研究旨在通过DBA/2 J和C57 BL/6 J近交系小鼠的药物辨别来广泛表征孕烷醇酮的行为效应。此外，本研究首次将孕烷醇酮的辨别性刺激效应与孕烷醇酮对时间表控制行为的运动效应进行比较，以充分了解神经类固醇孕烷醇酮的主观和运动行为效应。

DESCRIPTION (provided by applicant): Neurosteroids represent a novel class of endogenous compounds that have the ability to exert rapid, nongenomic effects and produce various endocrine and behavioral effects. The role of these steroids in the behavioral effects of ethanol has received much recent attention. In the behavioral assay of drug discrimination, neurosteroids such as allopregnanolone and pregnanolone produce discriminative stimuli that are very similar to ethanol. These behavioral effects of neurosteroids need to be thoroughly examined to understand the role of these endogenous compounds in ethanol's actions. Drug discrimination is commonly used to identify candidate receptor mechanisms underlying the behavioral effects of drugs. This research proposal aims to extensively characterize the behavioral effects of pregnanolone through drug discrimination in DBA/2J and C57BL/6J inbred mice. Also, this research for the first time will compare the discriminative stimulus effects of pregnanolone with the motor effects of pregnanolone on schedule-controlled behavior to obtain an adequate picture of the subjective and motor behavioral effects of the neurosteroid pregnanolone.