ANIMAL / PHYSIOLOGY CORE

动物/生理学核心（9002）为所有项目提供支持。每个项目都使用Fischer 344大鼠，这些大鼠由核心获得、圈养和饲养。核心提供了一个集中的最先进的屏障设施，用于圈养老年心脏研究中使用的无特定病原体大鼠。该中心为老年学研究所需的实验动物提供符合健康标准的动物护理，包括无病原体的环境、日常一般健康状况评估、体格检查以及体重和肾功能测量。核心位于Louis Stokes VAMC动物资源设施内的专用空间内。 核心组织提供的心脏模型为所有项目提供心脏组织。心脏组织从安乐死后立即切除的心脏获得，用于研究基线状态下的心脏;以及从分离的缓冲液灌注的心脏实验获得。心脏灌注方案包括时间控制灌注、单独缺血和长达120分钟的再灌注期。两个离体灌注心脏模型，Langendorff模型和工作心脏模型。修订后的申请中提出的研究使用了两种现有模型以及新方法。Core将灌注离体心脏，以制备组织，用于在0003前分离心肌细胞。 核心人员将与0003合作，将体内心脏基因转移的模型带到核心。该核心将促进项目间合作研究：阿米妥干预和谷氧还蛋白干预。这些研究最大限度地共享项目之间的组织。此外，核心协调四个项目之间的相互作用，以共享缺血前、缺血后和缺血后基线状态下未治疗大鼠的组织。 缺血和再灌注。 将心脏组织提供给core-9004，用于分离心脏线粒体并通过光学和电子显微镜进行分析。其他心脏组织直接提供给项目进行生物化学和分子研究。多余的心脏组织样本被分类并储存以备将来使用。实验被输入到一个共同的数据库，通过网络提供给所有项目。

The Animal/Physiology Core (9002) provides support to all projects. Each project uses Fischer 344 rats that are acquired, housed, and maintained by the Core. The Core provides a centralized state of the art barrier facility for the housing of specific pathogen free rats used in the studies of the aged heart. The Core provides animal care that complies with standards of health for experimental animals required for gerontological studies, including a pathogen free environment, assessment of general health status on a daily basis, physical examination and measurements of body weight and renal function. The Core is located within dedicated space within the Animal Resource Facility of the Louis Stokes VAMC. The cardiac models supplied by the Core provide cardiac tissue to all of the projects. Cardiac tissue is obtained from hearts excised immediately following euthanasia for the study of the heart in the baseline state; and from isolated, buffer perfused heart experiments. Heart perfusion protocols include time control perfusions, ischemia alone, and reperfusion periods of up to 120 minutes. Two isolated perfused heart models have been employed, the Langendorff model and the working heart model. The studies proposed in the revised application use the two current models as well as new approaches. The Core will perfuse isolated hearts to prepare tissue for the isolation of cardiac myocytes by 0003. Core personnel will collaborate with 0003 to bring the model of in vivo cardiac gene transfer to the Core. The Core will facilitate the Inter-project Collaborative Studies: Amytal-Intervention and the Glutaredoxin-Intervention. These studies maximize sharing of tissues among projects. In addition, the Core co-ordinates interactions among the four projects to share tissues from untreated rats in the baseline state prior to ischemia, following ischemia, and following ischemia and reperfusion. Cardiac tissue is provided to core-9004 for the isolation of cardiac mitochondria and for analysis by light and electron microscopy. Additional cardiac tissue is provided directly to the projects for biochemical and molecular studies. Excess cardiac tissue samples are catalogued and stored for future use. Experiments are entered into a common database available via network to all projects.