Animal Care Core

动物护理核心

• 批准号: 9325003
• 负责人: Kristin Nicole Grimsrud
• 金额: $ 17.37万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9325003
• 关键词: Acclimatization; Age; Animal Housing; Animal Model; Animals; Area; Bariatrics; Behavior; Behavioral; Biology; Blood specimen; Caring; Communicable Diseases; Containment; Cryopreservation; Data; Data Collection; Endocrinology; Enrollment; Ensure; Environment; Euthanasia; Evaluation; Exercise; Female; Gastrectomy; Gastric Bypass; Gender; Gene Targeting; Generations; Genetic; Genetic Engineering; Genetically Engineered Mouse; Glucose tolerance test; Gnotobiotic; Health; Health Services; Health Status; Housing; Human Resources; IACUC; In Vitro; Indwelling Catheter; Infusion procedures; Institution; Laboratories; Laboratory Animals; Maintenance; Metabolic; Microinjections; Microsurgery; Mission; Modeling; Monitor; Mus; Mutant Strains Mice; Operative Surgical Procedures; Phenotype; Procedures; Quarantine; Reproducibility; Research; Research Infrastructure; Research Personnel; Resources; Rodent; Sampling; Schedule; Services; Shipping; Ships; Specialist; Students; Surgical Models; Telemetry; Testing; Time; Training; Vendor; Veterinarians; Work; animal care; animation; authority; bariatric surgery; cognitive function; cohort; cost effective; environmental enrichment for laboratory animals; experience; feeding; genome editing; gut microbiome; implantation; in vivo; insulin tolerance; laboratory experience; laboratory facility; male; meetings; metabolic phenotype; microbiome; mouse model; mutant mouse model; programs; repository; sample collection

CORE B: ANIMAL CARE CORE ABSTRACT (CORE B) The Animal Care Core (Core B) provides project support for animal care and housing, importation, in vivo procedural sampling, and animal model creation for the MMPC-UCD. The Core accomplishes this through importation and acclimation of mice with minimal delay and reduced quarantine. The Core has access to and oversight of a dedicated in vivo Phenotyping Facility and associated state-of-the-art vivaria, including a high- containment “approved vendor-status” barrier vivarium. In addition, the Core has primary access to a new, UCD Gnotobiotic Mouse Research Center (GMRC). The Core provides numerous scientific support services as well, including rederivation and creation of genetically-modified mutant mice, re-animation of a variety of models cryopreserved within the KOMP or MMRRC repositories. These new and extant mouse models can then be bred to produce male and female cohorts for in vivo and in vitro phenotyping, including rederivation into the GMRC for gut microbiome studies. The Core also operates a Microsurgery Suite for creating surgically-manipulated mouse models, such as bariatric surgical models. The Core can produce for researchers cohorts of mice that have undergone Vertical Sleeve Gastrectomy (VSG) and Roux en Y Gastric Bypass (RYGB) bariatric procedures. Upon request from users, the Core can work with the other Phenotyping Cores to provide testing, manipulation, and sampling of these models. In addition, the Core can ship these surgically-manipulated models as well as any genetically-engineered mouse lines to requesting investigators from our approved vendor barrier vivarium, facilitating direct entry into the recipient institution's vivarium. This ensures minimal delay for investigators for receipt and use of these animal models at their institution. The Core also provides ancillary services and procedures, including blood sampling for in vivo testing for glucose tolerance tests and insulin tolerance tests, which are then sent to the Phenotyping Cores (e.g., Core C, Endocrinology and Metabolism Core) for analysis. The Core can also provide telemetry implantation and indwelling catheter placement for continuous blood sampling. The Core is supported by two trained laboratory animal veterinarians, three trained rodent surgical technicians, one import/export coordinator, and two phenotyping technicians. In these ways, the Animal Care Core is uniquely qualified to support the varied in vivo needs of MMPC-UCD users to meet their specific needs, from rapid importation and testing, genetically- engineered and genome-edited (Cas9) mouse lines, bariatric and other surgically-manipulated models, specialized housing under barrier conditions for direct shipping to investigators, and maintenance under gnotobiotic conditions for microbiome manipulation.

核心B：动物护理核心 摘要（核心B） 动物护理核心（核心B）为动物护理和住房、进口、体内 MMPC-UCD的程序采样和动物模型创建。核心通过以下方式实现这一点： 以最小延迟和减少隔离的方式进口和驯化小鼠。核心可以访问 监督专门的体内表型分析设施和相关的最先进的动物园，包括高 遏制“批准的供应商地位”屏障动物园。此外，核心还拥有一个新的、 UCD Gnotobiotic小鼠研究中心（GMRC）。核心提供众多科学支持服务 以及，包括转基因突变小鼠的重新衍生和创造，各种 在KOMP或MMRRC储存库中冷冻保存的模型。这些新的和现存的小鼠模型可以 然后进行繁殖以产生雄性和雌性群体，用于体内和体外表型分析，包括再衍生 用于肠道微生物组研究核心还经营一个显微手术套件， 外科操作的小鼠模型，如减肥手术模型。核心可以生产 研究人员对接受垂直袖状胃切除术（VSG）和Roux en Y胃切除术的小鼠进行了队列研究。 旁路（RYGB）减肥手术。根据用户的要求，核心可以与其他表型分析仪一起工作。 提供这些模型的测试、操作和采样的核心。此外，核心可以运送这些 动物操纵模型以及任何基因工程小鼠品系，以请求研究者 从我们批准的供应商屏障动物园，促进直接进入受体机构的动物园。这 确保研究者在其机构接收和使用这些动物模型的延迟最小化。的 核心还提供辅助服务和程序，包括血液采样的体内测试葡萄糖 耐受性测试和胰岛素耐受性测试，然后将其发送到表型核心（例如，核心C， 内分泌和代谢核心）进行分析。核心还可以提供遥测植入， 留置导管放置，用于连续采血。核心由两个训练有素的实验室支持 动物兽医，三名训练有素的啮齿动物外科技术员，一名进出口协调员，两名 表型分析技术人员。在这些方面，动物护理核心是唯一有资格支持各种各样的， MMPC-UCD用户的体内需求，以满足他们的特定需求，从快速进口和测试，遗传- 工程化和基因组编辑的（Cas9）小鼠系，肥胖症治疗和其他药物操纵的模型， 隔离墙条件下的专门住房，以便直接运送给调查人员， 用于微生物组操作的gnotobiotic条件。