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Creatine Transport Across the Blood Brain Barrier

肌酸跨血脑屏障转运

基本信息

批准号：
7062430
负责人：
JOSEPH Floyd CLARK
金额：
$ 14.99万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-15 至 2008-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7062430
关键词：
aminoacid transport bioenergetics blood brain barrier blood tests brain disorders brain metabolism cognition disorders creatine disease /disorder model drug design /synthesis /production drug screening /evaluation gene mutation genetically modified animals immunocytochemistry laboratory mouse model design /development nuclear magnetic resonance spectroscopy urinalysis

项目摘要

DESCRIPTION (provided by applicant): Recently, we described severe expressive and cognitive delays in a 6-year-old boy, who has a unique creatine (Cr) deficiency in the brain, which was diagnosed by proton magnetic resonance spectroscopy (MRS). Upon further analysis, we found that he has a nonsense mutation in the X-lined Cr transporter gene (CT1;SLC6A8), which resulted in the expression of a truncated (non-functional) Cr transporter protein. Since that study, four additional families have been recognized in Cincinnati with mutations in the X-linked Cr transporter gene and nearly 30 families worldwide. These patients all have mental retardation, severe expressive language disorder and mild epilepsy. Despite a growing body of knowledge about the Cr, Cr kinase and phosphocreatine system in the brain, there is no standardized method for improving brain function when the brain creatine transporter is deficient. What is clearly needed is a suitable animal model of this disease such that methods to get creatine across the blood brain barrier can be developed and tested. In this project we will develop a Cr transporter knockout mouse model, such that the efficacy of new treatment paradigms, drugs, and other therapies can be tested. For this research project, we propose to test the following hypotheses: 1) a mouse knockout of this Cr transport defect can model the human disease, and 2) that therapeutic strategies can be given to normalize brain function in these mice. This mouse knockout will model the Cr transporter defect we have discovered in that the brain will lack the ability to transport creatine across the blood brain barrier. To address Hypothesis 1, we will generate a Cr transporter knockout mouse. Cr levels will be determined in the brains of these mice and we will characterize the functional, and biochemical changes observed in Cr transporter knockout mice. Having an animal model that closely mirrors the human disease will enable adequate testing and development of therapies designed at getting creatine across the blood brain barrier and improving brain function. To address Hypothesis 2, the Cr transporter knockout mice and control mice will be treated with Cr formulations that may be capable of transporting creatine across the blood brain barrier and improve brain metabolism and cognitive function of the mice. The goal for Hypothesis 2 is to develop methods and drugs to improve brain energy metabolism by getting creatine into the brain.

描述（由申请人提供）：最近，我们描述了一名6岁男孩的严重表达和认知迟缓，他患有独特的脑肌酸（Cr）缺乏症，并通过质子磁共振波谱（MRS）诊断。通过进一步分析，我们发现他在x线Cr转运蛋白基因（CT1;SLC6A8）上有一个无义突变，导致一个截断的（无功能的）Cr转运蛋白表达。自那项研究以来，在辛辛那提又发现了四个携带x -连锁Cr转运基因突变的家族，在全世界发现了近30个家族。这些患者都有智力低下、严重的表达性语言障碍和轻度癫痫。尽管人们对脑中的Cr、Cr激酶和磷酸肌酸系统的了解越来越多，但当脑肌酸转运体缺乏时，还没有标准化的方法来改善脑功能。显然，我们需要的是一种适合这种疾病的动物模型，这样就可以开发和测试让肌酸穿过血脑屏障的方法。在这个项目中，我们将开发一个Cr转运蛋白敲除小鼠模型，这样新的治疗模式、药物和其他疗法的功效就可以得到测试。在本研究项目中，我们提出验证以下假设：1)小鼠敲除这种Cr转运缺陷可以模拟人类疾病，2)可以给予治疗策略使这些小鼠的脑功能正常化。这种小鼠基因敲除将模拟我们发现的Cr转运蛋白缺陷，即大脑将缺乏通过血脑屏障运输肌酸的能力。为了验证假设1，我们将产生一只Cr转运蛋白敲除小鼠。我们将测定这些小鼠大脑中的Cr水平，并描述在Cr转运蛋白敲除小鼠中观察到的功能和生化变化。拥有一个与人类疾病密切相关的动物模型，将有助于充分测试和开发旨在让肌酸通过血脑屏障并改善大脑功能的疗法。为了验证假设2，将Cr转运蛋白敲除小鼠和对照小鼠进行Cr配方治疗，该配方可能能够通过血脑屏障运输肌酸，并改善小鼠的脑代谢和认知功能。假设2的目标是开发方法和药物，通过让肌酸进入大脑来改善大脑能量代谢。