CORE--LABORATORY FACILITY

核心--实验室设施

• 批准号: 7553761
• 负责人: GUO-YUAN YANG
• 金额: $ 21.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7553761
• 关键词: angiogenesis; biomedical equipment resource; biomedical facility; blood vessel disorder; cerebral hemorrhage; disease /disorder model; experimental designs; genotype; laboratory mouse; method development

Core C, the Laboratory Core, will provide infrastructure and experimental methodology for this Program Project Grant application. Core C includes a small animal surgical lab, a cellular and molecular biology lab, and an imaging analysis lab. Key portions of the experiments within the PPG will be performed in the Core that will furnish quality control and consistency for the experiments. Aim 1 will provide a reproducible brain non-tumor angiogenesis (neo-vascularization) model in the mouse and the technology to deliver naked DNA constructs of interest to brain parenchyma. Project 3 (Boudreau) and Project 4 (Nishimura) will investigate the interaction of the homeobox genes and the integrins to develop abnormal cerebral microvessels, therefore, in vivo brain non-tumor angiogenesis model will provide a unique tool to examine whether the hypothesized mechanisms are rational. Aim 2 will organize and process human blood samples, extract DNA, and perform ApoE genotyping, which will support the study of genetic polymorphisms from Project 1 (Young). In addition, Core C will process genotyping for the transgenic mice study in Project 4 The Core will assist in the design of the experiments, the writing of experimental proposals, solving methodological problems, and will provide a forum for discussing and interpreting results.

Core C（实验室核心）将为该计划项目授予应用程序提供基础架构和实验方法。核心C包括一个小型动物外科实验室，一个细胞和分子生物学实验室以及成像分析实验室。 PPG内实验的关键部分将以核心进行，该核心将提供实验的质量控制和一致性。 AIM 1将在小鼠和技术中提供可再现的大脑非肿瘤血管生成（新血管化）模型，并为脑实质提供了感兴趣的裸露DNA构建体。项目3（Boudreau）和Project 4（Nishimura）将研究同源蛋白基因和整联蛋白的相互作用，以开发异常的大脑微血管，因此，在体内脑非肿瘤血管生成模型将提供独特的工具来研究假设机制是否是基化的。 AIM 2将组织和处理人类血液样本，提取DNA并执行APOE基因分型，这将支持项目1（Young）的遗传多态性的研究。此外，Core C将处理项目4中转基因小鼠研究的基因分型，核心将有助于设计实验，实验建议的撰写，解决方法论问题，并为讨论和解释结果提供论坛。