CORE--LABORATORY FACILITY

Core C，实验室核心，将为该计划项目拨款申请提供基础设施和实验方法。核心C包括一个小动物外科实验室，一个细胞和分子生物学实验室，以及一个成像分析实验室。PPG内实验的关键部分将在核心中进行，这将为实验提供质量控制和一致性。目的1将在小鼠中提供可重复的脑非肿瘤血管生成（新血管生成）模型，以及将感兴趣的裸DNA构建物传递到脑实质的技术。Project 3 （Boudreau）和Project 4 （Nishimura）将研究同源盒基因与整合素的相互作用对异常脑微血管的形成，因此，体内脑非肿瘤血管生成模型将为检验假设机制是否合理提供独特的工具。目标2将组织和处理人类血液样本，提取DNA，并进行ApoE基因分型，这将支持项目1 （Young）的遗传多态性研究。此外，Core C将为Project 4中的转基因小鼠研究进行基因分型，Core将协助设计实验，撰写实验建议，解决方法问题，并将提供讨论和解释结果的论坛。

Core C, the Laboratory Core, will provide infrastructure and experimental methodology for this Program Project Grant application. Core C includes a small animal surgical lab, a cellular and molecular biology lab, and an imaging analysis lab. Key portions of the experiments within the PPG will be performed in the Core that will furnish quality control and consistency for the experiments. Aim 1 will provide a reproducible brain non-tumor angiogenesis (neo-vascularization) model in the mouse and the technology to deliver naked DNA constructs of interest to brain parenchyma. Project 3 (Boudreau) and Project 4 (Nishimura) will investigate the interaction of the homeobox genes and the integrins to develop abnormal cerebral microvessels, therefore, in vivo brain non-tumor angiogenesis model will provide a unique tool to examine whether the hypothesized mechanisms are rational. Aim 2 will organize and process human blood samples, extract DNA, and perform ApoE genotyping, which will support the study of genetic polymorphisms from Project 1 (Young). In addition, Core C will process genotyping for the transgenic mice study in Project 4 The Core will assist in the design of the experiments, the writing of experimental proposals, solving methodological problems, and will provide a forum for discussing and interpreting results.