Biomimetic Modular Design for Advanced Biomaterials

先进生物材料的仿生模块化设计

• 批准号: 7039193
• 负责人: ZHIBIN GUAN
• 金额: $ 26.66万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7039193
• 关键词: bioengineering /biomedical engineering; biomaterial compatibility; biomaterial development /preparation; biomimetics; hydrogen bond; peptide analog; polymers; protein folding

DESCRIPTION (provided by applicant): The proposed research investigates a biomimetic modular polymer design as a new strategy to achieve advanced biomaterials. The broad, long-term objective of this research is to develop rational design of biomaterials having high order structures for advanced properties. A specific challenge in biomaterials research is to design a polymer that has a combination of mechanical strength, fracture toughness, and elasticity - three fundamental mechanical properties that are highly desired but usually exclusive to each other in polymeric materials. Many structural biopolymers, such as the muscle protein titin, employ modular domain structures to achieve the combination of these three fundamental mechanical properties in one system. We propose to mimic the modular domain design in synthetic biomaterials. Specifically, we propose to synthesize and investigate biomimetic modular polymers having the following modules: (1) quadruple hydrogen bonding modules, (2) peptidomimetic beta-sheet modules, and (3) small protein modules. Our hypothesis is that the introduction of well-defined modular domain structures into synthetic biopolymers should lead to biomaterials having a combination of mechanical strength, toughness, and elasticity. Whereas numerous biomedical applications can be envisioned for this type of biomaterial, this proposal is focused on developing model polymers having modular domain structures with which to study the fundamental structure-property correlation in synthetic biomaterials. Through the proposed studies the following specific aims will be accomplished: (1) Synthesis and studies on discrete oligomers and polymers using well defined quadruple hydrogen bonding modules; (2) Synthesis and studies on discrete oligomers and polymers containing peptidomimetic a-sheet modules; (3) Synthesis and studies on discrete oligomers and polymers using protein G domain III (PG3) as module; and (4) Systematic investigation on the mechanical properties of the discrete oligomers as well as the high mass polymers made from the above modules at single-molecule, nanoscopic and macroscopic levels.

描述（由申请人提供）：拟议的研究调查了仿生模块化聚合物设计作为实现先进生物材料的新策略。这项研究的广泛，长期的目标是开发具有高级性能的高阶结构的生物材料的合理设计。生物材料研究中的一个具体挑战是设计一种具有机械强度、断裂韧性和弹性的组合的聚合物-这三种基本机械性能是高度期望的，但通常在聚合物材料中彼此排斥。许多结构生物聚合物，如肌肉蛋白肌联蛋白，采用模块化结构域结构，以实现这三个基本的机械性能在一个系统中的组合。我们建议在合成生物材料中模仿模块化结构域设计。具体而言，我们建议合成和研究具有以下模块的仿生模块聚合物：（1）四重氢键模块，（2）拟肽β-折叠模块，和（3）小蛋白质模块。我们的假设是，将定义明确的模块化结构域结构引入合成生物聚合物中，应该会导致生物材料具有机械强度、韧性和弹性的组合。虽然许多生物医学应用可以设想这种类型的生物材料，该建议的重点是开发具有模块化域结构的模型聚合物，研究合成生物材料中的基本结构-性能相关性。通过这些研究，我们将完成以下具体目标：（1）以四重氢键为结构单元的离散寡聚体和聚合物的合成与研究;（2）以拟肽α-折叠结构单元为结构单元的离散寡聚体和聚合物的合成与研究;（3）以蛋白G结构域Ⅲ（PG 3）为结构单元的离散寡聚体和聚合物的合成与研究;（4）在单分子、纳米和宏观水平上系统地研究了由上述模块制备的离散低聚物和高质量聚合物的力学性能。