Delivering Mechanistic Understanding and Novel Approaches to Native Chemical Ligation

提供对天然化学连接的机制理解和新方法

• 批准号: 2646511
• 金额: --
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2646511
• 关键词: Delivering; Mechanistic; Understanding; Novel; Approaches

Native chemical ligation (NCL) is a chemical reaction which is critical in the synthesis of proteins. It has enabled chemists to prepare highly potent peptide-based pharmaceuticals for a range of diseases. Despite its ubiquity, aspects of the reaction's mechanism are still debated, so brute-force reaction screening is commonly employed, which is an expensive and time-consuming process. We will use a quantitative, combined synthetic-kinetic approach to elucidate the mechanism for NCL across a range of amino acids. Using this insight, we aim to understand current limitations and drawbacks of NCL in order to deliver better and more efficient synthetic procedures.

天然化学连接（NCL）是一种在蛋白质合成中至关重要的化学反应。它使化学家能够为一系列疾病制备高效的肽基药物。尽管它无处不在，但反应机制的各个方面仍然存在争议，因此通常采用蛮力反应筛选，这是一个昂贵且耗时的过程。我们将使用定量的，结合合成动力学的方法来阐明跨一系列氨基酸的NCL的机制。利用这一见解，我们的目标是了解NCL目前的局限性和缺点，以提供更好，更有效的合成程序。