Molecular view of transcription activation & repression

描述（由申请人提供）：真核生物中的转录调控依赖于与基因启动子中的特定序列相互作用的因子。这是一个高度调节和微调的过程，对细胞生长、分化和发育以及对环境变化的反应至关重要。它在很大程度上由调节因子之间的负性和正性蛋白质-蛋白质相互作用控制，但对它们的了解甚少。在原子水平上理解这些相互作用是理解转录调控机制的关键。酵母GAL系统，作为转录调控的范例，提供了一个独特的机会，检查这些相互作用在原子水平上，特别是在光的遗传信息的财富。这项建议的目的是研究参与转录开关的因子的相互作用和分子特征，并阐明激活结构域的特征，这在很大程度上仍然是个谜。 相关性：真核生物的转录调控是一个高度调控和精细调节的过程，对细胞生长、分化和发育以及对环境变化的响应至关重要。我们的目标是了解这种重要类型的调节如何在非常详细的原子水平上起作用。

DESCRIPTION (provided by applicant): Transcriptional regulation in eukaryotes relies on factors that interact with specific sequences in gene promoters. This is a highly regulated and finely tuned process, which is critical to cell growth, differentiation and development and response to environmental changes. It is largely governed by negative and positive protein-protein interactions between regulatory factors, yet they are poorly understood. Understanding these interactions at the atomic level is key to understanding the mechanism of how transcriptional regulation works. The yeast GAL system, which serves as a paradigm for transcriptional regulation, provides a unique opportunity to examine these interactions at an atomic level, especially in light of the wealth of genetic information available. The objective of this proposal is to study the interactions and molecular characteristics of the factors involved in a transcriptional switch, and to shed light on the characteristics of activation domains, which remain largely enigmatic. Relevance: Transcriptional regulation in eukaryotes is a highly regulated and finely tuned process, which is critical to cell growth, differentiation and development and response to environmental changes. Our objective is to understand how this important type of regulation works on a very detailed atomic level.