A molecular view of transcriptional activation and repression

转录激活和抑制的分子观点

• 批准号: 7569017
• 负责人: Leemor Joshua-Tor
• 金额: $ 30.86万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7569017
• 关键词: Affect; Affinity; Binding; Biological Assay; Calorimetry; Characteristics; Complex; Development; Dimerization; Eukaryota; Galactose; Genes; Genetic; Hand; In Vitro; Knowledge; Light; Molecular; Mutation; Nature; Peptides; Phage Display; Process; Property; Regulation; Repression; Structure; System; Thermodynamics; Transcriptional Activation; Transcriptional Regulation; Transducers; Variant; Work; Yeasts; base; cell growth; design; environmental change; in vivo; mutant; promoter; protein protein interaction; research study; response

Transcriptional regulation in eukaryotes relies on factors that interact with specific sequences in gene promoters. This is a highly regulated and finely tuned process, which is critical to cell growth, differentiation and development and response to environmental changes. It is largely governed by negative and positive protein-protein interactions between regulatory factors, yet they are poorly understood. Understanding these interactions at the atomic level is key to understanding the mechanism of how transcriptional regulation works. The yeast GAL system, which serves as a paradigm for transcriptional regulation, provides a unique opportunity to examine these interactions at an atomic level, especially in light of the wealth of genetic information available. The objective of this proposal is to study the interactions and molecular characteristics of the factors involved in a transcriptional switch, and to shed light on the characteristics of activation domains, which remain largely enigmatic. Relevance: Transcriptional regulation in eukaryotes is a highly regulated and finely tuned process, which is critical to cell growth, differentiation and development and response to environmental changes. Our objective is to understand how this important type of regulation works on a very detailed atomic level.

真核生物中的转录调控依赖于与基因中特定序列相互作用的因子 发起人。这是一个高度调节和精细调节的过程，对细胞生长、分化 发展和应对环境变化。它在很大程度上是由消极和积极的 调节因子之间的蛋白质-蛋白质相互作用，但他们知之甚少。了解这些 原子水平上的相互作用是理解转录调控机制的关键， 工程.酵母GAL系统作为转录调控的范例，提供了独特的 有机会在原子水平上研究这些相互作用，特别是鉴于丰富的遗传物质， information available.本提案的目的是研究相互作用和分子特征 转录开关中涉及的因子，并阐明激活的特征 这些领域在很大程度上仍然是谜。 相关性：真核生物中的转录调控是一个高度调节和微调的过程， 对细胞生长、分化和发育以及对环境变化的反应至关重要。我们的目标 是要了解这种重要的调节方式如何在非常详细的原子水平上起作用。