Apoptosis and Renewal of Neural Progenitor Cells

神经祖细胞的凋亡和更新

• 批准号: 7034192
• 负责人: PASKO RAKIC
• 金额: $ 34.2万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7034192
• 关键词: Parkinson' s disease; amyotrophic lateral sclerosis; apoptosis; calcium flux; cerebral ischemia /hypoxia; enzyme activity; enzyme induction /repression; genetically modified animals; gerbil /jird; glutamates; isozymes; laboratory mouse; laboratory rat; methylphenyltetrahydropyridine; nervous system transplantation; neural degeneration; neurotoxicology; neurotoxins; oxidative stress; protein kinase; stress proteins; superoxide dismutase; tissue /cell culture; transplant rejection; xenotransplantation

DESCRIPTION (provided by applicant): This proposal is a competitive renewal application for our research program on the role of the Caspase family of proteases, the c-Jun NH2-terminal kinase (JNK), and the phosphatidylserine receptor (PSR) in neuronal apoptosis in brain development and neurological diseases. In the present project, we will extend our research of these signaling pathways with a new emphasis on the mechanism of apoptosis and renewal of neural progenitor cells. Aim 1: We will investigate the mechanism downstream of phosphatidylserine and phosphatidylserine receptor (PS-PSR) recognition leading to the apoptosis of neural progenitor cells. We will express PS on the outer cell surface of neural progenitor cells and examine the signaling events following PS-PSR recognition. In addition, we will test whether the disruption of PS-PSR recognition in the embryonic brain will alter the apoptosis of neural progenitor cells. Aim 2: We will examine the interaction between the JNK and canonical Wnt/beta-catenin signaling in the decision of self-renewal or cell-cycle exit by neural progenitor cells. We will determine whether the canonical Wnt/beta-catenin pathway mediates self-renewal, and whether JNK activation attenuates the canonical Wnt/ beta-catenin signaling to promote differentiation of the daughter cells. Aim 3: We will use a transgenic system to calculate the net increase of adult-generated neurons in the mouse brain. In addition, we will test whether EGF and FGF2 infusion can accelerate adult neurogenesis and attract newly born neurons into stroke damaged brain areas. In summary, the present project is built on our research of JNK, phosphatidylserine receptor (PSR), and canonical Wnt/beta-catenin signaling with an emphasis on the mechanism governing the adjustment of the number of neural progenitor cells (Aim 1), the decision of cell-cycle exit by neural progenitor cells (Aim 2), and the accelerated neurogenesis after brain injury (Aim 3). These studies will enhance our understanding of neural plasticity and functional recovery after brain injury.

描述(由申请人提供)：这项提案是我们关于Caspase家族、c-jun氨基末端激酶(JNK)和磷脂酰丝氨酸受体(PSR)在脑发育和神经系统疾病中神经细胞凋亡中作用的研究计划的竞争性续签申请。在本项目中，我们将扩展我们对这些信号通路的研究，重点放在神经前体细胞的凋亡和更新机制上。目的1：探讨磷脂酰丝氨酸和磷脂酰丝氨酸受体(PS-PSR)识别下游导致神经前体细胞凋亡的机制。我们将在神经前体细胞的外表面表达PS，并检测PS-PSR识别后的信号事件。此外，我们还将测试胚胎脑中PS-PSR识别的中断是否会改变神经前体细胞的凋亡。目的2：我们将研究JNK和典型的Wnt/β-catenin信号在神经前体细胞决定自我更新或细胞周期退出中的相互作用。我们将确定规范的Wnt/β-catenin途径是否介导自我更新，以及JNK激活是否减弱规范的Wnt/β-catenin信号以促进子代细胞的分化。目的3：我们将使用转基因系统来计算小鼠大脑中成人产生的神经元的净增加量。此外，我们将测试注射EGF和FGF2是否可以加速成人神经发生，并吸引新生的神经元进入中风受损的脑区。综上所述，本项目建立在我们对JNK、磷脂酰丝氨酸受体(PSR)和典型的Wnt/β-连环蛋白信号转导机制的研究基础上，重点研究了神经前体细胞数量的调节机制(目标1)，神经前体细胞决定细胞周期退出的机制(目标2)，以及脑损伤后神经再生的加速(目标3)。这些研究将加深我们对脑损伤后神经可塑性和功能恢复的理解。