Multi-objective de novo protein design

多目标从头蛋白质设计

• 批准号: 2721830
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2721830
• 关键词: Multi; objective; de; novo; protein

De novo protein design involves generating protein sequences that fold to a desired structure. This endeavour encompasses numerous applications across the healthcare and the fine chemistry industries: formulating and optimising protein therapeutics that bind drug targets, generating artificial proteins that elicit immune response against a specific antigen, developing enzyme catalysts for industrial applications, or constructing protein-based nanostructures that can deliver drugs more efficiently. While the field of protein design has experienced significant progress in the past decade, one salient challenge it has faced is the limited control over the physicochemical properties of the generated designs, which invariably leads to multiple iterations of the design-make-test experimental cycle. The objective of this doctoral project is to fill this knowledge gap by developing deep learning algorithms to design proteins with specific physicochemical properties. This project falls within the EPSRC digital healthcare research area, and more generally under the artificial and intelligence research theme.The project comprises three independent work packages.- Protein property optimization algorithms. The first work package will expand the property prediction models developed in the summer rotation into a property engineering platform. The property prediction algorithms will be extended from thermodynamic stability into other properties such as solubility and post-translational modifications. The candidate will explore multi-objective optimization algorithms to enable targeted improvement of the physicochemical properties of a protein. This package includes experimentation across different deep learning architectures, starting from the initial combination of graph convolutional neural networks to other architectures incorporating neural attention.- De novo protein design. The next stage of the project will address the problem of simultaneously generating a new, physically plausible three-dimensional structure for a protein, and an amino acid sequence that encodes these features. The candidate will develop generative models, starting from the currently existing literature in diffusion models and hallucination networks, and condition them on predicted physicochemical properties such as stability and solubility to ensure that the generated designs are optimal for development at a protein production facility. The candidate and her supervisors are currently in conversations with the Woolfson Lab in the University of Bristol who are keen to provide data and validate generated designs experimentally. However, the project's success is not dependent on this collaboration.- Multi-objective protein design. The last part of the project will focus on multi-objective protein design i.e. designing protein sequences that fulfil a variety of conditions. The candidate will combine her understanding of protein property prediction and de novo protein design to generate artificial proteins, like vaccines and antibodies, that pass a performance profile on multiple properties like immunogenicity, thermostability, toxicity, half-life etc. This part of the project will integrate the machine learning architectures developed by the candidate in previous years, as well as other models generated within the ecosystem of the Oxford Protein Informatics Group.

从头蛋白质设计涉及产生折叠成所需结构的蛋白质序列。这一努力涵盖了医疗保健和精细化学行业的众多应用：制定和优化结合药物靶点的蛋白质治疗剂，产生引发针对特定抗原的免疫反应的人工蛋白质，开发用于工业应用的酶催化剂，或构建可以更有效地递送药物的蛋白质纳米结构。虽然蛋白质设计领域在过去十年中取得了重大进展，但它面临的一个突出挑战是对生成的设计的物理化学性质的有限控制，这总是导致设计-制造-测试实验周期的多次迭代。这个博士项目的目标是通过开发深度学习算法来设计具有特定物理化学性质的蛋白质来填补这一知识空白。该项目福尔斯属于EPSRC数字医疗保健研究领域，更广泛地说，属于人工和智能研究主题。该项目包括三个独立的工作包。蛋白质性质优化算法。第一个工作包将把夏季轮调开发的房地产预测模型扩展为房地产工程平台。性质预测算法将从热力学稳定性扩展到其他性质，如溶解度和翻译后修饰。候选人将探索多目标优化算法，以实现蛋白质物理化学性质的有针对性的改善。该软件包包括不同深度学习架构的实验，从图卷积神经网络的初始组合到其他包含神经注意力的架构。从头蛋白质设计。该项目的下一阶段将解决同时生成蛋白质的新的物理上合理的三维结构和编码这些特征的氨基酸序列的问题。候选人将从扩散模型和幻觉网络的现有文献开始开发生成模型，并根据预测的物理化学性质（如稳定性和溶解度）对其进行调节，以确保生成的设计对于蛋白质生产设施的开发是最佳的。这位候选人和她的导师目前正在与布里斯托大学的伍尔夫森实验室进行对话，他们热衷于提供数据并通过实验验证生成的设计。然而，该项目的成功并不依赖于这种合作。多目标蛋白质设计该项目的最后一部分将侧重于多目标蛋白质设计，即设计满足各种条件的蛋白质序列。候选人将联合收割机结合她对蛋白质性质预测和从头蛋白质设计的理解，以生成人工蛋白质，如疫苗和抗体，这些蛋白质通过免疫原性，热稳定性，毒性，半衰期等多个性质的性能特征。以及牛津蛋白质信息学小组生态系统内生成的其他模型。