Anticancer Potential of Black Cohosh on Breast Cancer

黑升麻对乳腺癌的抗癌潜力

• 批准号: 7011261
• 负责人: LINDA S EINBOND
• 金额: $ 10.01万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7011261
• 关键词: alternative medicine; angiosperms; antineoplastics; apoptosis; athymic mouse; breast neoplasms; cell cycle; cell growth regulation; cell line; drug screening /evaluation; medicinal plants; pharmacokinetics; plant extracts; viral carcinogenesis; western blottings

DESCRIPTION (provided by applicant): My intent is to combine my background in microbiology and viral oncology with newer interests in cancer and herbal medicines. My objective is to initiate a productive career in research and teaching at a university or research institute that will lead to the development and clinical use of novel anticancer therapies and chemopreventive agents. The focus of this project will be mechanistic studies of herbal medicines, in particular black cohosh, on cancer in vitro and in vivo. The ideal mentor to accomplish this is Dr. I. Bernard Weinstein, who is an expert on molecular aspects of carcinogenesis and the ideal environment is Columbia University College of Physicians and Surgeons. Black cohosh, Actaea racemosa L. (= Cimicifuga racemosa), is a North American perennial in the buttercup family (Ranunculaceae) used for various women's health conditions. The overall objective of this project is to determine whether black cohosh contains components that may eventually be useful in the prevention or treatment of breast cancer. Indeed, our preliminary experiments indicate that black cohosh extracts inhibit the growth of human breast cancer cells. We will determine the pharmacokinetics of black cohosh. Partially purified extracts and specific chemicals will be examined for their effects, alone and in combination with chemotherapy agents, on the function of specific molecules that control cell growth, the cell cycle, apoptosis and the function of the ER and Her-2 receptor. Western blot analysis will be performed to examine possible effects on the expression of cyclin D1 related proteins. Luciferase reporter assays will be done to monitor effects on the transcription of NF-kB, fos, myc, jun and PPAR-g. Analytic chemical techniques will be used to identify novel compounds that might mediate these effects. To ascertain the efficacy of the compounds to treat humans, we will examine effects of an extract of black cohosh on xenografts of human breast cancer cell lines in athymic mice. This research project could identify new approaches to breast cancer prevention and therapy.

描述（由申请人提供）：我的目的是将我的微生物学和病毒肿瘤学背景与对癌症和草药的新兴趣相结合。我的目标是在大学或研究所的研究和教学领域发起富有成效的职业，这将导致新颖的抗癌疗法和化学预防剂的开发和临床使用。该项目的重点将是对草药的机理研究，尤其是黑色同病的体外和体内癌症。实现这一目标的理想导师是I. Bernard Weinstein博士，他是癌变分子方面的专家，理想的环境是哥伦比亚大学医师和外科医生学院。 Black Cohosh，Actaea racemosa L.（= Cimicifuga Racemosa），是用于各种妇女健康状况的毛cup家族（Ranunculaceae）的北美多年生。该项目的总体目的是确定黑色cohosh是否包含最终可能有用的组成部分，这些组成部分可用于预防或治疗乳腺癌。实际上，我们的初步实验表明，黑色同胞提取物抑制了人类乳腺癌细胞的生长。我们将确定黑色cohosh的药代动力学。将检查部分纯化的提取物和特定的化学物质，单独检查其作用，并与化学疗法剂结合使用，对控制细胞生长，细胞周期，凋亡以及ER和Her-2受体功能的特定分子功能。将进行蛋白质印迹分析，以检查对细胞周期蛋白D1相关蛋白的表达的可能影响。将进行荧光素酶报告基因测定，以监视对NF-KB，FOS，MYC，JUN和PPAR-G转录的影响。分析化学技术将用于鉴定可能介导这些作用的新型化合物。为了确定化合物治疗人类的功效，我们将检查黑卷提取物对无胸腺小鼠人类乳腺癌细胞系的异种移植物的影响。该研究项目可以确定预防乳腺癌和治疗的新方法。