Identification of PAS kinase activators

描述（由申请人提供）：细胞已经进化出复杂的机制来感知其营养环境并相应地调节生长和增殖。未能适当协调细胞代谢导致各种人类疾病和综合征。PAS激酶通过感知细胞代谢状态和调节糖代谢，在协调代谢中起关键作用。PAS激酶是一种丝氨酸/苏氨酸蛋白激酶，由小分子结合PAS结构域顺式调节。虽然生物学上相关的配体是未知的，但已经确定了两种刺激物导致S.酿酒酵母：第一，在不可发酵的碳源中生长，第二，细胞完整性胁迫。生物化学和遗传技术将被用来确定参与PAS激酶激活的因素，在这两种刺激。这些知识将有助于理解PAS激酶如何协调细胞生长和增殖，以应对环境营养和压力。

DESCRIPTION (provided by applicant): Cells have evolved complex mechanisms to sense their nutritional environment and regulate growth and proliferation accordingly. Failure to properly coordinate cellular metabolism results in a variety of human diseases and syndromes. PAS kinase plays a critical role in coordinating metabolism by sensing cellular metabolic status and regulating sugar metabolism. PAS kinase is a serine/threonine protein kinase regulated in cis by a small molecule-binding PAS domain. Although the biologically relevant ligand is unknown, two stimuli have been identified that lead to activation of PAS kinase in S. cerevisiae: first, growth in non-fermentable carbon sources and, second, cell integrity stress. Both biochemical and genetic techniques will be used to identify factors involved in PAS kinase activation in response to both stimuli. This knowledge will aid in understanding how PAS kinase coordinates cell growth and proliferation in response to environmental nutrients and stresses.