Mechanisms of Olfactory Interneurnon Differentiation

描述（由申请人提供）：本提案的目的是描述来自脑室下区干细胞生态位的哺乳动物成年嗅球中间神经元的基本特征和作用。两个特定的目的已经发展，以确定早期出生的中间神经元是否不同于成人产生的中间神经元，并确定活动对成人来源的中间神经元分化的影响。具体目的1：为了确定胚胎和围产期神经元发育过程中分化的嗅球中间神经元是否与来自成年神经元前体的神经元不同，将进行遗传命运定位实验。特异性目标2：表征活动对成人来源的嗅球中间神经元的影响，中间神经元形态、基因表达和存活将在基因消融活动后进行评估。这些实验将提供关于成年神经发生对成熟动物功能的意义和贡献的重要信息。此外，关于成人神经元分化机制的信息将有助于引导疾病或损伤后神经系统修复的分化。

DESCRIPTION (provided by applicant): The objective of this proposal is to characterize the essential characteristics and role of mammalian, adult-born olfactory bulb interneurons derived from the subventricular zone stem cell niche. Two specific aims have been developed to determine if early-born interneurons differ from those generated in the adult, and to determine the influence of activity on the differentiation of adult-derived interneurons. Specific Aim 1: In order to determine whether olfactory bulb interneurons differentiating during embryonic and perinatal neuronal development are different from those derived from adult neuronal precursors, genetic fate-mapping experiments will be performed. Specific Aim 2: To characterize the influence of activity on adult-derived olfactory bulb interneurons, interneuron morphology, gene expression, and survival will be assessed following genetic ablation of activity. These experiments will contribute important information regarding the significance and contribution of adult neurogenesis to the function of the mature animal. In addition, information regarding the mechanism of neuronal differentiation in the adult will aid in efforts to guide that differentiation toward nervous system repair following disease or injury.