Mechanisms of Olfactory Interneurnon Differentiation

嗅觉中间神经元分化的机制

• 批准号: 7367163
• 负责人: Jennie Leigh Close
• 金额: $ 4.88万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7367163
• 关键词: Ablation; Address; Adult; Affect; Afferent Neurons; Animals; Anterior nares; Cells; Characteristics; Development; Disease; Disruption; Embryo; Enhancers; Gene Expression; Genetic; Imagery; Injury; Interneurons; Knock-in Mouse; Label; LacZ Genes; Location; Maps; Morphology; Mus; Neuraxis; Neuronal Differentiation; Neurons; Nose; Numbers; Perinatal; Population; Potassium Channel; Recruitment Activity; Role; Rosa; Staging; Stem cells; Tamoxifen; Time; cell type; critical developmental period; granule cell; mature animal; neurogenesis; olfactory bulb; olfactory marker protein; olfactory receptor; olfactory stimulus; postnatal; promoter; recombinase; repaired; research study; transcription factor

The objective of this proposal is to characterize the essential characteristics, and role of, mammalian, adult- born olfactory bulb interneurons derived from the subventricular zone stem cell niche. Therefore, two specific aims have been developed to determine if early-born interneurons differ from those generated in the adult, and to determine the influence of activity on the differentiation of adult-derived interneurons. Specific Aim 1: In order to determine whether olfactory bulb interneurons differentiating during embryonic and perinatal neuronal development are different from those derived from adult neuronal precursors, genetic fate-mapping experiments will be performed. Specific Aim 2: To characterize the influence of activity on adult-derived olfactory bulb interneurons, interneuron morphology, gene expression, and survival will be assessedfollowing genetic ablation of activity. These experiments will contribute important information regarding the significance and contribution of adult neurogenesis to the function of the mature animal. In addition, information regarding the mechanism of neuronal differentiation in the adult will aid in efforts to guide that differentiation toward nervoussystem repair following disease or injury.

这项建议的目的是描述哺乳动物、成体-- 新生的嗅球中间神经元来源于脑室下区干细胞巢。因此，有两个具体的 目的是确定早期出生的中间神经元是否不同于成年产生的中间神经元， 并确定活性对成体来源的中间神经元分化的影响。 具体目标1：为了确定嗅球中间神经元在胚胎期间是否分化 而围产期神经元的发育不同于来自成年神经元前体的发育，遗传 将进行命运映射实验。 具体目的2：研究活动对成年嗅球中间神经元的影响。 中间神经元的形态、基因表达和存活率将在基因消融活性后进行评估。 这些实验将提供有关成人的意义和贡献的重要信息。 神经发生对成熟动物功能的影响。此外，有关该机制的信息 成人的神经元分化将有助于引导神经系统的分化 疾病或受伤后的修复。