Elucidation of the Histone 3' End Formation Mechanism

组蛋白 3 末端形成机制的阐明

• 批准号: 7096570
• 负责人: ERIC J WAGNER
• 金额: $ 5.04万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7096570
• 关键词: Drosophilidae; HeLa cells; gene mutation; green fluorescent proteins; histones; messenger RNA; nucleic acid metabolism; postdoctoral investigator; posttranscriptional RNA processing; protein binding; protein protein interaction; protein purification; small interfering RNA; transcription factor

DESCRIPTION (provided by applicant): The broad long-term objectives to this proposal are to identify new factors involved in histone mRNA 3' end formation both biochemically and genetically and to characterize those proteins in vivo and in vitro. Specific Aim 1 is designed to use a combination of classical and innovative biochemical techniques to identify and purify proteins binding in a functionally relevant manner to histone pre-mRNA in HeLa nuclear extract. Specific Aim 2 will involve the use of a novel GFP based genetic screen to be performed in Drosophila melanogaster aimed at identifying loss of function mutants in the process. This screen will be performed to near saturating levels with the aid of an embryo sorter. Specific Aim 3 is to develop an in vivo system to study the function relevance of identified factors as well as to characterize them. This in vivo assay may also be applied in a novel siRNA-based genetic screen in mammalian cells. An in vitro system will also be developed to study histone mRNA 3' end formation in the context of active transcription. Histone mRNA 3' end formation is intimately related to the cell cycle and DNA replication, thus understanding this process may lead to the production of more effective chemotherapeutic agents.

描述（由申请人提供）：

项目成果

Expression of an RNAi-resistant SLBP restores proper S-phase progression.

RNAi 抗性 SLBP 的表达可恢复正常的 S 期进展。

• DOI: 10.1042/bst0330471
• 发表时间: 2005
• 期刊: Biochemical Society transactions.
• 作者: Wagner,EJ;Berkow,A;Marzluff,WF
• 通讯作者: Marzluff,WF