Characterization of Novel miRNAs During Development

开发过程中新型 miRNA 的表征

• 批准号: 7067103
• 负责人: Aurora Esquela Kerscher
• 金额: $ 5.04万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7067103
• 关键词: Caenorhabditis elegans; RNA; antisense nucleic acid; biochemical evolution; developmental genetics; embryo /fetus; gene induction /repression; genetic manipulation; genetically modified animals; helminth genetics; in situ hybridization; laboratory mouse; mature animal; northern blottings; postdoctoral investigator; species difference

DESCRIPTION (provided by applicant): MicroRNAs belong to a novel class of gene regulatory molecules found in plants and animals that control gene expression by binding to complimentary sequences on target mRNAs. Hundreds of miRNAs have recently been identified in worm, fly, and mammalian genomes. The function of only five miRNAs has been elucidated in animals and all five control important developmental processes. The uncharacterized miRNAs may also act as important gene regulators during development. Thirty C. elegans miRNAs are temporally regulated during development, eleven of which share homology to mammalian miRNAs. The hypothesis to be tested is that conserved temporally expressed miRNAs essential for C. elegans development will also be important during mammalian development. This proposal will 1) characterize the expression patterns of six novel conserved and temporally regulated miRNAs in C. elegans 2) determine the role of select conserved and temporally regulated miRNAs during C. elegans development and 3) investigate the function of one or two promising temporally regulated miRNA homologues in the mouse. These studies will contribute to the understanding of gene regulation and development and may lead to valuable insights on human disease.

描述（由申请人提供）：

项目成果

The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells.

• DOI: 10.1038/onc.2009.106
• 发表时间: 2009-06-25
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Kato, M.;Paranjape, T.;Ullrich, R.;Nallur, S.;Gillespie, E.;Keane, K.;Esquela-Kerscher, A.;Weidhaas, J. B.;Slack, F. J.
• 通讯作者: Slack, F. J.