PRMT9, A Novel Protein Arginine Methyltransferase

PRMT9，一种新型蛋白质精氨酸甲基转移酶

• 批准号: 7155226
• 负责人: Cecilia Zurita
• 金额: $ 2.97万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2010-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7155226
• 关键词: arginine; enzyme activity; green fluorescent proteins; methylation; methyltransferase; northern blottings; posttranslational modifications; predoctoral investigator; protein localization; protein structure function; radiotracer; tissue /cell culture

DESCRIPTION (provided by applicant): Each of the nine current members of the protein arginine methyltransferase (PRMT) family has substrates whose differential methylations can lead to distinct physiological changes in the cell. However, although many in vivo substrates have been identified, many others have not. For example, the substrates of PRMT2 have not been characterized due to lack of measurable activity. Cellular roles that have been established in arginine methylation include the involvement of RNA splicing, processing and transport, transcriptional controls, DNA repair, and intracellular signaling. This proposal will set forth specific aims to characterize the newest member of the methyltransferase family, PRMT9. The first step would be to determine enzyme specificity of methylable substrates. Once the substrates are identified, and PRMT9 is characterized by its type of methyltransferase activity, I will determine its localization and its tissue expression pattern. These results will contribute to our understanding of the overall significance of methyltransferases in cellular processes.

描述（由申请人提供）：蛋白精氨酸甲基转移酶（PRMT）家族的九个当前成员中的每个成员都具有底物，其差异甲基化可以导致细胞中的不同生理变化。 但是，尽管已经确定了许多体内底物，但其他许多人却没有。 例如，由于缺乏可测量的活性，未表征PRMT2的底物。 精氨酸甲基化中已确定的细胞作用包括RNA剪接，加工和运输，转录控制，DNA修复和细胞内信号传导的参与。 该提案将制定特定的目的，以表征甲基转移酶家族的最新成员PRMT9。 第一步是确定可甲基底物的酶特异性。 一旦鉴定出底物，PRMT9的特征是其类型的甲基转移酶活性，我将确定其定位及其组织表达模式。 这些结果将有助于我们理解甲基转移酶在细胞过程中的总体意义。