Peptide Based Antibacterial Coating

肽基抗菌涂层

基本信息

批准号：
6993335
负责人：
Jennifer Ann Neff
金额：
$ 9.95万
依托单位：
ALLVIVO VASCULAR, INC.
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-20 至 2006-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6993335
关键词：
antiinfective agents biomaterial development /preparation biotechnology surface coating synthetic peptide

项目摘要

DESCRIPTION (provided by applicant): The long-term objective of this SBIR Phase I project is to develop antibacterial coatings for medical devices. The use of traditional antimicrobials on devices poses a substantial risk for developing drug resistant bacteria. The objective is to develop coatings that work by a different mode of action that are not likely to support the rise of resistant microorganisms. Antibacterial coatings will be produced by linking an antibacterial peptide, nisin, to surfaces via a flexible tether. Recent studies of this peptide's behavior suggest antimicrobial activity could be maintained when bound to a surface if sufficient solvent accessibility and molecular mobility are preserved. A coating of the peptide prepared in this way should be safe, functional, and long-lasting. In Phase I, chemically-modified peptide derivatives will be covalently bound to immobilized, end-group activated poly(ethylene oxide) chains. The distribution of surface tethered nisin will be quantified, and antibacterial properties of free and immobilized peptide-polymer constructs will be evaluated using a sensitive indicator strain of bacteria. The stability and duration of activity of tethered peptides will be measured in the presence of plasma. In Phase II, coating activity will be tested against clinically significant bacteria strains and in a model of central venous catheter infection in rats. This research will be conducted in close cooperation with Oregon State University thereby bringing together two important technologies that will be required for success. The collaboration will assimilate the strength of DV Biomed's industrial R&D experience in surface coating technology with OSU's unique basic science experience with the antibacterial peptide. Commercial applications for these coatings include: catheters, medical, optical and dental devices as well as research tools to investigate the mechanism of action and structural requirements for activity of antibacterial peptides. Medical device-related infections are a major problem and antibacterial coatings are needed to prevent illness, morbidity and excess cost. However, current antibacterial coatings support the rise of drug resistant bacteria, which is an important medical concern. The aim of this work is to produce alternative coatings based on natural compounds that kill bacteria. The use of such compounds in medical device coatings is expected to provide a safe, effective alternative to antibacterial drugs that will enable broader use. If successful, these coatings will improve patient care by decreasing their risk for developing hard to treat infections and will be an important milestone in the effort to control the rise of drug resistant bacteria.

描述（由申请人提供）：该SBIR I期项目的长期目标是开发用于医疗设备的抗菌涂料。在设备上使用传统抗菌剂会带来耐药细菌的重大风险。目的是开发通过不同的作用方式起作用的涂料，这些涂层不太可能支持耐药微生物的兴起。将通过将抗菌肽Nisin连接到通过柔性系绳将抗菌涂料与表面相连而产生的抗菌涂层。对该肽行为的最新研究表明，如果保留足够的溶剂可及性和分子迁移率，则可以保持抗菌活性。以这种方式制备的肽的涂层应该是安全，功能性和持久的。在第一阶段，化学改性的肽衍生物将共价结合到固定的，最终组激活的聚（氧化乙烷）链。将对表面束缚的尼我们的表面分布进行量化，并将使用敏感的细菌指示菌株评估游离和固定肽聚合物构建体的抗菌特性。在存在血浆的情况下，将测量系绳肽活性的稳定性和持续时间。在II期中，将针对临床上显着的细菌菌株和大鼠中央静脉导管感染模型进行涂料活性。这项研究将与俄勒冈州立大学密切合作，从而将成功所需的两种重要技术汇总在一起。该合作将通过OSU在抗菌肽中独特的基础科学经验来吸收DV Biomed在表面涂料技术中的工业研发经验的实力。这些涂料的商业应用包括：导管，医疗，光学和牙科设备以及研究抗菌肽活动的作用机理和结构要求的研究工具。与医疗设备相关的感染是一个主要问题，需要抗菌涂料来预防疾病，发病率和过量成本。但是，当前的抗菌涂层支持耐药细菌的兴起，这是一个重要的医学问题。这项工作的目的是基于杀死细菌的天然化合物生产替代涂料。预计在医疗设备涂料中使用此类化合物将为抗菌药物提供安全，有效的替代品，可以更广泛使用。如果成功的话，这些涂层将通过降低难以治疗感染的风险来改善患者护理，这将是控制耐药细菌兴起的重要里程碑。