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Peptide Based Antibacterial Coating

肽基抗菌涂层

基本信息

批准号：
6993335
负责人：
Jennifer Ann Neff
金额：
$ 9.95万
依托单位：
ALLVIVO VASCULAR, INC.
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-20 至 2006-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6993335
关键词：
antiinfective agents biomaterial development /preparation biotechnology surface coating synthetic peptide

项目摘要

DESCRIPTION (provided by applicant): The long-term objective of this SBIR Phase I project is to develop antibacterial coatings for medical devices. The use of traditional antimicrobials on devices poses a substantial risk for developing drug resistant bacteria. The objective is to develop coatings that work by a different mode of action that are not likely to support the rise of resistant microorganisms. Antibacterial coatings will be produced by linking an antibacterial peptide, nisin, to surfaces via a flexible tether. Recent studies of this peptide's behavior suggest antimicrobial activity could be maintained when bound to a surface if sufficient solvent accessibility and molecular mobility are preserved. A coating of the peptide prepared in this way should be safe, functional, and long-lasting. In Phase I, chemically-modified peptide derivatives will be covalently bound to immobilized, end-group activated poly(ethylene oxide) chains. The distribution of surface tethered nisin will be quantified, and antibacterial properties of free and immobilized peptide-polymer constructs will be evaluated using a sensitive indicator strain of bacteria. The stability and duration of activity of tethered peptides will be measured in the presence of plasma. In Phase II, coating activity will be tested against clinically significant bacteria strains and in a model of central venous catheter infection in rats. This research will be conducted in close cooperation with Oregon State University thereby bringing together two important technologies that will be required for success. The collaboration will assimilate the strength of DV Biomed's industrial R&D experience in surface coating technology with OSU's unique basic science experience with the antibacterial peptide. Commercial applications for these coatings include: catheters, medical, optical and dental devices as well as research tools to investigate the mechanism of action and structural requirements for activity of antibacterial peptides. Medical device-related infections are a major problem and antibacterial coatings are needed to prevent illness, morbidity and excess cost. However, current antibacterial coatings support the rise of drug resistant bacteria, which is an important medical concern. The aim of this work is to produce alternative coatings based on natural compounds that kill bacteria. The use of such compounds in medical device coatings is expected to provide a safe, effective alternative to antibacterial drugs that will enable broader use. If successful, these coatings will improve patient care by decreasing their risk for developing hard to treat infections and will be an important milestone in the effort to control the rise of drug resistant bacteria.

描述（由申请人提供）：SBIR第一阶段项目的长期目标是开发医疗器械的抗菌涂层。在器械上使用传统抗菌剂会造成产生耐药性细菌的重大风险。目的是开发通过不同的作用模式起作用的涂层，这些作用模式不太可能支持耐药微生物的兴起。抗菌涂层将通过将抗菌肽（乳链菌肽）通过柔性系链连接到表面来产生。最近对这种肽的行为的研究表明，如果保持足够的溶剂可及性和分子流动性，则当结合到表面时可以保持抗微生物活性。以这种方式制备的肽的涂层应该是安全的、功能性的和持久的。在第一阶段，化学修饰的肽衍生物将共价结合到固定的，端基活化的聚（环氧乙烷）链。将定量表面拴系的乳链菌肽的分布，并且将使用细菌的敏感指示菌株来评价游离的和固定化的肽-聚合物构建体的抗菌特性。将在血浆存在下测量栓系肽的稳定性和活性持续时间。在第II阶段，将针对具有临床意义的细菌菌株和大鼠中心静脉导管感染模型检测涂层活性。这项研究将与俄勒冈州州立大学密切合作，从而汇集了两个重要的技术，将需要成功。此次合作将吸收DV Biomed在表面涂层技术方面的工业研发经验与OSU在抗菌肽方面的独特基础科学经验。这些涂层的商业应用包括：导管，医疗，光学和牙科设备以及研究工具，以研究抗菌肽活性的作用机制和结构要求。医疗器械相关的感染是一个主要问题，需要抗菌涂层来防止疾病，发病率和超额成本。然而，目前的抗菌涂层支持耐药细菌的增加，这是一个重要的医学问题。这项工作的目的是生产基于天然化合物的替代涂层，以杀死细菌。在医疗器械涂层中使用此类化合物有望为抗菌药物提供安全、有效的替代品，从而实现更广泛的应用。如果成功，这些涂层将通过降低患者发生难以治疗的感染的风险来改善患者护理，并将成为控制耐药细菌增加的重要里程碑。