Nanodroplet PCR Lab-Chip for Clinical Pathogen Detection

用于临床病原体检测的纳米液滴 PCR 实验室芯片

• 批准号: 6935579
• 负责人: MICHAEL G POLLACK
• 金额: $ 16.55万
• 依托单位: ADVANCED LIQUID LOGIC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935579
• 关键词: biomedical equipment development; clinical biomedical equipment; communicable disease diagnosis; computer assisted diagnosis; microfluidics; nanotechnology; polymerase chain reaction; portable biomedical equipment; rapid diagnosis

DESCRIPTION (provided by applicant): Rapid and accurate detection and identification of pathogens is crucial for the effective management and treatment of infectious disease. While molecular diagnostic techniques including PCR are a promising approach for rapid and sensitive detection of pathogens, they suffer from a number of technical drawbacks which have slowed their acceptance in the clinic. Microfluidic lab-on-a-chip technologies are well-poised to overcome many of these limitations by providing vastly increased levels of automation, throughput and reliability while reducing assay costs and instrument sizes. Our long term goal is to develop a highly-flexible lab-on-a-chip platform for detection of pathogens by real-time PCR based on our innovative nanoliter droplet-based "digital microfluidic" concept. Ultimately, we imagine that this platform would enable a new generation of highly-automated handheld instruments capable of providing fast, accurate, and multiplexed molecular test results for the diagnosis of infectious disease. Towards this goal, in phase I, we will demonstrate the feasibility of performing parallel automated real-time PCR reactions on our platform. We will develop a prototype chip capable of automatically setting-up nanodroplet reactions in a fully-programmable and combinatorial fashion. We will further demonstrate that thin-film heaters can be integrated directly into the chip to perform the thermocycling operations. In later phases the detector and control electronics would also be miniaturized or integrated onto the chip. Additionally, in phase II we propose to apply our prototype device to the problem of Candida detection in extremely low-birth-weight infants and rigorously validate our technology through participation in a multi-center clinical study in collaboration with Duke University.

描述（由申请人提供）：快速准确地检测和鉴定病原体对于有效管理和治疗传染病至关重要。虽然包括PCR的分子诊断技术是用于快速和灵敏地检测病原体的有前途的方法，但是它们遭受许多技术缺陷，这些缺陷减缓了它们在临床中的接受。微流控芯片实验室技术通过提供大大提高的自动化水平、吞吐量和可靠性，同时降低分析成本和仪器尺寸，很好地克服了许多这些限制。我们的长期目标是开发一个高度灵活的芯片实验室平台，用于基于我们创新的基于纳升液滴的“数字微流体”概念通过实时PCR检测病原体。最终，我们想象这个平台将使新一代高度自动化的手持仪器能够为传染病的诊断提供快速，准确和多重的分子检测结果。为了实现这一目标，在第一阶段，我们将证明在我们的平台上进行并行自动化实时PCR反应的可行性。我们将开发一种原型芯片，能够以完全可编程和组合的方式自动设置纳米液滴反应。我们将进一步证明，薄膜加热器可以直接集成到芯片中，以执行热循环操作。在以后的阶段中，检测器和控制电子设备也将被小型化或集成到芯片上。此外，在第二阶段，我们建议将我们的原型设备应用于极低出生体重婴儿的念珠菌检测问题，并通过与杜克大学合作参与多中心临床研究来严格验证我们的技术。