Miniaturized DNA Sequencer for Identification of Microbial Pathogens

用于微生物病原体鉴定的小型化 DNA 测序仪

• 批准号: 7747020
• 负责人: MICHAEL G POLLACK
• 金额: $ 17.84万
• 依托单位: ADVANCED LIQUID LOGIC
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7747020
• 关键词: Academic Medical Centers; Base Pairing; Biochemistry; Chemistry; Clinical; Collaborations; Communicable Diseases; Core Facility; DNA; DNA Sequence; DNA amplification; Development; Devices; Diagnosis; Diagnostic; Discrimination; Equipment; Evaluation; Goals; Immunoassay; Laboratories; Left; Medical; Methods; Methylation; Microfluidics; Molds; Patients; Performance; Pharmacogenetics; Phase; Preparation; Protocols documentation; Research; Research Personnel; Sampling; Specialist; Synthesis Chemistry; System; Technology; Testing; Yeasts; abstracting; base; cancer type; clinical application; clinically relevant; cost; design; digital; improved; instrument; meetings; microbial; miniaturize; pathogen; prototype; public health relevance; research clinical testing; tool development

DESCRIPTION (provided by applicant): Miniaturized DNA Sequencer for Identification of Microbial Pathogens Abstract. The goal of this project is develop a compact and inexpensive DNA sequencer based on the implementation of sequencing-by-synthesis chemistry on a droplet-based digital microfluidic cartridge. The proposed device would be capable of sequencing 10's to 100's of base pairs using an inexpensive disposable cartridge and a compact and simple piece of equipment. The cartridge will also integrate sample preparation capability, including DNA amplification, to enable widespread use by non-specialists and provide rapid and reliable results at low cost. The initial application for this technology will be microbial pathogen identification. The rationale for this is that even a few tens of base pairs can provide discrimination of microbial pathogens while there is a genuine clinical and medical need for an instrument that can perform this type of analysis rapidly, automatically, and at low cost. As a proof of concept, a prototype system will be developed in Phase I and an evaluation will be performed to assess the ability of the prototype to identify a set of clinically relevant yeasts and moulds. PUBLIC HEALTH RELEVANCE: A compact and inexpensive instrument to sequence small amounts of DNA for the purpose of identifying microbial pathogens will be developed and tested. The proposed instrument would improve the ability of clinicians to diagnose and treat infectious diseases.

描述（由申请人提供）：用于鉴定微生物病原体的小型化DNA测序仪摘要。这个项目的目标是开发一种紧凑和廉价的DNA测序仪，基于基于液滴的数字微流控盒上的合成化学测序的实现。所提出的设备将能够测序10到100个碱基对，使用廉价的一次性药筒和一个紧凑和简单的设备。该试剂盒还将整合样品制备能力，包括DNA扩增，使非专业人员能够广泛使用，并以低成本提供快速可靠的结果。这项技术的最初应用将是微生物病原体鉴定。这样做的理由是，即使几十个碱基对也可以提供微生物病原体的区分，而真正的临床和医疗需要一种能够快速、自动和低成本地进行这种分析的仪器。作为概念验证，将在第一阶段开发原型系统，并进行评估，以评估原型识别一组临床相关酵母和霉菌的能力。