Lactoferrin prevents biofilm formation at wound

乳铁蛋白可防止伤口处生​​物膜的形成

• 批准号: 6933304
• 负责人: NING HUANG
• 金额: $ 10万
• 依托单位: VENTRIA BIOSCIENCE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933304
• 关键词: Pseudomonas aeruginosa; Staphylococcus aureus; affinity chromatography; antibacterial agents; antibiotics; bacterial disease; biofilm; biotechnology; biotherapeutic agent; disease /disorder prevention /control; drug resistance; drug screening /evaluation; enzyme linked immunosorbent assay; genetically modified plants; growth inhibitors; high throughput technology; lactoferrin; lysozyme; peptide chemical synthesis; protein purification; recombinant proteins; rice; wound healing

DESCRIPTION (provided by applicant): Wound healing is a major health issue for the elderly. Biofilm development at the wound site renders many existing treatments ineffective in treating wound pathogens because when bacteria is protected by biofilm it increases its resistance to antibiotics. Bacteria protected by biofilm demonstrate a 100 to 1000 fold increase in resistance to antibiotics. Recent research has shown that human lactoferrin is effective in preventing biofilm formation. Production of recombinant human lactoferrin (rhLF) in rice grain provides the foundation for large quantities of raw material at an affordable cost. A rhLF-based treatment to enhance wound healing is proposed. In phase I, rice grain will be scaled up and a process to efficiently purify lactoferrin from rice grain will be developed. This procedure will be used to prepare quality rhLF, which would then be used to test rhLF's effect on biofilm formation for various bacterial strains isolated from wound site including antibiotic resistant strains.

描述（由申请人提供）：伤口愈合是老年人的主要健康问题。伤口部位的生物膜发育使许多现有治疗方法无效地治疗伤口病原体，因为当细菌受到生物膜保护时，它会增加其对抗生素的抗性。受生物膜保护的细菌表明，对抗生素的耐药性增加了100至1000倍。最近的研究表明，人乳铁铁蛋白可有效预防生物膜形成。水稻谷物中重组人乳铁蛋白（RHLF）的产生为大量原材料提供了基础。提出了基于RHLF的治疗方法，以增强伤口愈合。在第一阶段，将扩大水稻颗粒，并将开发有效纯化乳铁蛋白的过程。该过程将用于制备优质的RHLF，然后将其用于测试RHLF对从伤口部位分离的各种细菌菌株（包括抗生素耐药菌株）的生物膜形成的影响。