Electrochemical Multispecific Molecular Detection System

描述（由申请人提供）： 我们打算建立一个快速，简单和廉价的无PCR分子检测系统，以满足生物防御诊断的需要。一旦开发出来，该系统将能够使用基本的电化学设备在几分钟内一步检测出可能的病毒和细菌性质的生物战病原体，以及抗生素耐药性。该研究将针对优先生物战病原体。第一阶段提出了一种新的诊断方法的可行性研究，该方法利用超薄导电聚合物膜直接电化学检测核酸，重点关注DMA和RNA检测的灵敏度和特异性，以创建替代目前使用的基于PCR的方法。避免PCR扩增的可能性将大大减少诊断时间和总诊断成本，使该系统可用于即时检测。

DESCRIPTION (provided by applicant): We intend to create a rapid, simple and inexpensive PCR-free molecular detection system to address biodefence diagnostic needs. Once developed, the system will be capable to detect possible biowarfare pathogens of viral and bacterial nature, together with antibiotic resistance, in minutes in one step using basic electrochemical equipment. The research will target priority biowarfare pathogens. Phase I presents a feasibility study for a novel diagnostic approach utilizing direct electrochemical detection of nucleic acids with ultra thin conductive polymer films, which will focus on sensitivity and specificity of DMA and RNA detection to create an alternative to currently used PCR-based methods. The possibility to avoid PCR amplification will considerably reduce diagnostic time and total diagnostic costs, making the system affordable for the point-of care testing.