SUBSTANCE P-SPECIFIC CONDUCTING POLYMER ELECTRODE

描述：神经活性多肽是多种人类 对疼痛、应激、出血、输血、直立和缺氧的反应，以及 正在影响免疫系统中一种特定神经肽的浓度 身体的某个系统可以为我们提供关于 健康或疾病的状态。目前，还没有针对神经肽的研究。 电极可用。 我们建议开发一种对P物质a进行特异性传感的电极 神经肽参与中枢神经内源性痛觉控制 系统。我们将使用一种新的电化学配准方法 电导率的变化，发生在专门设计的导电性 聚合物基质对固定化聚合物基质的协同作用 选择神经肽的抗体。这种电极专一性可以 通过选择固定化抗体的特异性进行设计。第一阶段将是 致力于P物质特定电极的研制和校准 在试管中。第一阶段将致力于电极的小型化和在 活体应用。具有设计的特异性的这种电极阵列将 能够检测到同一部位的几种多肽神经递质。 建议的商业应用： 根据我们的方法开发的电极将是一个重大的进步 在检测多肽神经递质方面，将是一种更好的替代 目前使用的免疫分析方法。使用这样的电极阵列 有了不同的特异性，就有可能检测到几种多肽神经递质。 在体内和同一地点，这将允许研究相互作用的行动 神经肽对神经内分泌系统的影响。

DESCRIPTION: Neuroactive peptides are the key players in a variety of human responses to pain, stress, hemorrhage, transfusion, orthostasis and anoxia, and are affecting the immune system The concentration of a specific neuropeptide in a certain system of the body can provide us with critical information on the state of health or disease. At present, there are no neuropeptide-specific electrodes available. We propose to develop an electrode for specific sensing of Substance P, a neuropeptide involved in the endogenous pain control in the central nervous system. We will be utilizing a novel approach of electrochemical registration of the conductivity changes, occurring in specifically designed conductive polymer matrix upon cooperative interaction of the immobilized on the matrix antibodies with selected neuropeptide. Specificity of such electrode can be designed by choosing the specificity of immobilized antibody. Phase I will be dedicated to the development and calibration of Substance P specific electrode in vitro. Phase I will be dedicated to miniaturization of the electrode and in vivo applications. An array of such electrodes with designed specificity will be able to detect several peptide neurotransmitters from the same site. PROPOSED COMMERCIAL APPLICATION: Electrodes, developed based on our approach, will represent a significant advance in the detection of peptide neurotransmitters, and will be a far better alternative to the immunoassays, which are currently used. Using an array of such electrodes with different specificity, it will be possible to detect several peptide neurotransmitters in vivo and from the same site, which will allow to study the interactive actions of neuropeptides on the neuroendocrine system.