A Tissue Engineered Meniscus Replacement

组织工程半月板替代物

• 批准号: 7148110
• 负责人: CHARLES J GATT
• 金额: $ 17.11万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7148110
• 关键词: articular cartilage; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial evaluation; biomaterial interface interaction; biomechanics; biotechnology; collagen; crosslink; density; disease /disorder model; dogs; histology; immunocytochemistry; joint prosthesis; knee; laboratory rabbit; mechanical stress; medical implant science; musculoskeletal regeneration; orthopedics; osteoarthritis; polymers; tensile strength; tissue engineering; tissue support frame; transmission electron microscopy

DESCRIPTION (provided by applicant): Arthroscopic removal of the meniscus is the most commonly performed orthopedic surgical procedure in the United States. However, it is accepted that the medial and lateral menisci of the human knee play a significant role in force transmission across the knee joint, and that these structures add to the stability of the human knee joint. Long term follow up of total and sub-total meniscectomy demonstrates the early onset of degenerative arthritis of the knee. The long term goal of this study is to design a tissue engineered scaffold that can be implanted at the site of a meniscal resection to prevent the onset of degenerative arthritis associated with meniscectomy. A prototype meniscal replacement, fabricated as a collagen sponge reinforced with biocompatible polymer fibers, has been developed in our laboratory. The hypothesis of this feasibility study is that variation of synthetic fiber density and pattern of distribution in a chemically crosslinked collagen sponge will enhance the mechanical properties, biologic response, and in vivo performance of a tissue engineered meniscus replacement. The specific aims of this study that will test the hypothesis are to: Analyze the effects of fiber density and distribution on the mechanical properties of a tissue engineered meniscus scaffold. Analyze the effects of fiber density and distribution on the biological response to a tissue engineered meniscus scaffold. Choose two scaffolds based on results from mechanical and biological analyses and perform a preliminary in vivo evaluation of the device in a canine model.

描述(申请人提供)：在美国，关节镜下切除半月板是最常见的骨科手术。然而，人们普遍认为膝关节的内侧半月板和外侧半月板在整个膝关节的力传递中起着重要作用，并且这些结构增加了人类膝关节的稳定性。半月板全切术和半月板次全切术的长期随访显示，膝关节退行性关节炎发病较早。这项研究的长期目标是设计一种组织工程支架，可以植入半月板切除的部位，以防止与半月板切除相关的退行性关节炎的发生。我们实验室研制了一种半月板替代物的原型，它是由生物相容性聚合物纤维增强的胶原海绵制成的。这项可行性研究的假设是，化学交联的胶原海绵中合成纤维密度和分布模式的变化将增强组织工程化半月板替代物的机械性能、生物响应和体内性能。这项研究的具体目的是检验这一假设：分析纤维密度和分布对组织工程半月板支架力学性能的影响。分析纤维密度和分布对组织工程半月板支架生物反应的影响。根据力学和生物学分析的结果选择两种支架，并在犬模型中对该装置进行初步的活体评估。