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High-Modulus Polyurethanes for Bone Tissue Engineering

用于骨组织工程的高模量聚氨酯

基本信息

批准号：
7140481
负责人：
Aaron Sanford Goldstein
金额：
$ 12.71万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-08-01 至 2008-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7140481
关键词：
bioengineering /biomedical engineering biomaterial compatibility biomaterial development /preparation bone bone marrow cell differentiation cell proliferation chemical synthesis laboratory rat osteoblasts perfusion polyurethanes stromal cells tissue /cell culture tissue engineering tissue support frame

项目摘要

DESCRIPTION (provided by applicant): Ex vivo manufacture of clinically effective engineered bone requires the incorporation of bioactive factors into a biomaterial scaffold that will stimulate vascular infiltration, tissue integration and remodeling in vivo. Polyurethanes are a class of durable biocompatible polymers well suited for numerous biomedical applications. We hypothesize that we can use known structure-property relationships to synthesize a family of novel high modulus, degradable, biocompatible segmented polyurethanes that are capable of supporting bone tissue formation. Concurrently, bone marrow stromal cells (BMSCs) are a class of adult stem cells that readily form mature bone in vivo and can be directed ex vivo to differentiate and secrete a bone like extracellular matrix. We hypothesize that by culturing BMSCs in porous polyurethane scaffolds using novel perfusion strategies to deliver nutrients and oxygen and activate mechanotransductive pathways, we can direct osteoblastic maturation and synthesis of those bioactive factors necessary to stimulate healing in vivo. The goals of the project are: 1) Synthesize a family of high modulus (10-500 MPa) segmented polyurethanes from biocompatible and biologically-derived precursors. 2) Demonstrate biocompatibility, degradability and processibility of polyurethanes into high modulus (0.1 to 5 MPa) porous foam scaffolds. 3) Develop a bone-like tissue ex vivo and determine the effect of polyurethane scaffold modulus and perfusion regimen on ex vivo bone formation. The high-risk innovations of this project are 1) a family of degradable polyurethanes tuned for bone tissue engineering applications, 2) a methodology to process polyurethanes into high porosity, interconnected pore foams with high compressive modulus, and 3) a strategy of intermittent perfusion culture that enhances ex vivo bone tissue development. The results of this project will be a family of engineered bone tissues to be tested in vivo, and methodologies and strategies to construct the next generation of engineered bone tissue.

描述（由申请人提供）：体外制造临床有效的工程骨需要将生物活性因子结合到生物材料支架中，该支架将刺激血管浸润，组织整合和体内重塑。聚氨酯是一类耐用的生物相容性聚合物，非常适合于许多生物医学应用。我们假设我们可以利用已知的结构-性能关系来合成一系列新型高模量、可降解、生物相容性的分段聚氨酯，这些聚氨酯能够支持骨组织的形成。同时，骨髓基质细胞（BMSCs）是一类在体内容易形成成熟骨的成体干细胞，可以在体外定向分化和分泌骨样细胞外基质。我们假设，通过在多孔聚氨酯支架中培养骨髓间充质干细胞，使用新的灌注策略来输送营养和氧气，并激活机械传导途径，我们可以指导成骨细胞成熟和合成那些促进体内愈合所必需的生物活性因子。该项目的目标是：