Elucidating the role of host cell ncRNAs in viral replication

阐明宿主细胞 ncRNA 在病毒复制中的作用

• 批准号: 2739507
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2739507
• 关键词: Elucidating; role; host; cell; ncRNAs

Non-coding RNAs (ncRNAs) are critical regulators of gene expression and their importance is emphasised as ncRNAs are frequently altered in many human diseases. Viruses have evolved ways to manipulate host ncRNAs to enhance virus gene expression and modulate the host response to infection. However, how and why ncRNA levels are altered during infection is yet to be fully elucidated.This project will investigate a novel mechanism utilised by viruses to dysregulate a recently identified group of ncRNAs, termed tRFs. The project will elucidate how and why this emerging ncRNA species are altered by the virus and also determine if reversing this dysregulation can be used as a novel antiviral strategy.The project is highly novel utilising a multidisciplinary approach and cutting-edge methodology including CLASH, RNA-IPs, imaging, cell biology and 3D tumour models.In summary, this project will identify novel ways viruses manipulate the host cell to enhance their replication and provide a better understanding of how ncRNA species can regulate gene expression and how this impacts on human disease. Moreover, it may provide new strategies for therapeutic interventions of important human pathogens.

非编码rna （ncRNAs）是基因表达的关键调控因子，其重要性被强调，因为ncRNAs在许多人类疾病中经常发生改变。病毒已经进化出操纵宿主ncrna的方法来增强病毒基因表达和调节宿主对感染的反应。然而，在感染过程中，ncRNA水平如何以及为何发生改变尚不完全清楚。该项目将研究病毒利用的一种新机制来失调最近发现的一组被称为trf的ncrna。该项目将阐明这种新兴的ncRNA物种是如何以及为什么被病毒改变的，并确定逆转这种失调是否可以作为一种新的抗病毒策略。该项目采用多学科方法和尖端方法，包括CLASH， RNA-IPs，成像，细胞生物学和3D肿瘤模型，非常新颖。总之，该项目将确定病毒操纵宿主细胞以增强其复制的新方法，并更好地了解ncRNA物种如何调节基因表达以及这如何影响人类疾病。此外，它可能为重要人类病原体的治疗干预提供新的策略。