Analyses of sodium bioenergetics in Vibrio cholerae

霍乱弧菌钠生物能学分析

• 批准号: 7103161
• 负责人: Claudia C Hase
• 金额: $ 34.95万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7103161
• 关键词: Vibrio cholerae; bacteria; bioenergetics; genes; physiology; role; sodium; virulence

DESCRIPTION (provided by applicant): All living cells establish transmembrane electrochemical gradients with the help of primary ion pumps. Primary Na+ pumps have been discovered in many microorganisms of quite diverse phylogenic groups and a transmembrane circulation of Na+ ions may play a significant role in the physiology of several bacteria. The recent completion of many bacterial genome sequences revealed the presence of genes encoding a variety of sodium-dependent systems in many organisms, including some that were not known to have a primary sodium cycle of energy. This indicates that these bacteria can utilize Na+ as a coupling ion instead of, or in addition to, the H+ cycle. Although little is known about the role of the sodium cycle of energy in the physiology of Vibrio cholerae, the presence of a multitude of sodium-dependent systems encoded in the genome of this organism merits the investigation of its various Na+ pumps. In the present application we will construct and analyze defined mutants in Na+-extruding enzymes in V. cholerae as a model organism to gain further insights into this very complex and important part of bacterial physiology. We propose a comprehensive and systematic analysis of the components that contribute to bacterial sodium bioenergetics by using extensive genetic manipulations in combination with sophisticated bioenergetic measurements. Results of the proposed study will not only enhance our understanding of the general bioenergetic pathways in bacteria, but will form a solid basis for future investigations, by ourselves and others, of the molecular devices maintaining ion homeostasis in microorganisms. Sodium bioenergetics probably plays a role in both the environmental and pathogenic phases of the V. cholerae life cycle. Thus, our research has the potential for generating fundamental perspectives related to bacterial physiology and will be of value to understanding basic biological concepts related to V. cholerae ecology in the environment and in the lumen of hosts that will be applicable to a variety of bacterial species.

描述（由申请人提供）：所有活细胞在一次离子泵的帮助下建立跨膜电化学梯度。原生Na+泵已在许多不同系统发育类群的微生物中被发现，Na+离子的跨膜循环可能在一些细菌的生理中起重要作用。最近完成的许多细菌基因组序列揭示了在许多生物体中存在编码各种钠依赖系统的基因，包括一些不知道具有初级钠能量循环的基因。这表明这些细菌可以利用Na+作为偶联离子，而不是H+循环，或者除了H+循环之外。虽然我们对霍乱弧菌生理中钠离子循环的作用知之甚少，但这种生物体基因组中编码的大量钠依赖系统值得对其各种钠离子泵进行研究。在目前的应用中，我们将构建和分析霍乱弧菌中Na+挤出酶的定义突变体，作为模式生物，以进一步了解细菌生理学中这一非常复杂和重要的部分。我们提出了一个全面和系统的组件，有助于细菌钠生物能量分析通过使用广泛的遗传操作结合复杂的生物能量测量。这项研究的结果不仅将增强我们对细菌中一般生物能量途径的理解，而且将为我们自己和其他人未来研究微生物中维持离子稳态的分子装置奠定坚实的基础。钠生物能量学可能在霍乱弧菌生命周期的环境阶段和致病阶段都起作用。因此，我们的研究有可能产生与细菌生理学相关的基本观点，并将对理解环境和宿主管腔中与霍乱弧菌生态学相关的基本生物学概念具有价值，这些概念将适用于各种细菌物种。